Chiara D'ATtilia
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Chiara D'ATtilia has a total of 18 co-authors. Here are the top co-authors by number of shared publications:
- Alberico Di Pinto
National Research Council (CNR), Institute of Molecular Biology and Pathology (IBPM), 00185 Rome, Italy
1 shared publication - Barbara Felici
Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy
1 shared publication - Danuše Tarkowská
Laboratory of Growth Regulators, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, v.v.i., Olomouc, Czech Republic
1 shared publication - Fabio D'Orso
Council for Agricultural Research and Economics, Research Centre for Genomics and Bioinformatics (CREA-GB), 00178 Rome, Italy
1 shared publication - Floriana Augelletti
Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy
1 shared publication - Francesco Sestili
Department of Agriculture and Forest Sciences (DAFNE), University of Tuscia, 01100 Viterbo, Italy
1 shared publication
Latest Publications
- Open Access
- 7 October 2026
- Published by Elsevier in Plant Physiology and Biochemistry
- Vol. 239
CRISPR-Cas9 knockout of IBH1 fine-tunes brassinosteroid metabolism and modulates plant architecture and grain traits in durum wheat
- 7 October 2026
- Published by Elsevier in Plant Physiology and Biochemistry
- Vol. 239
Open AccessCRISPR-Cas9 knockout of IBH1 fine-tunes brassinosteroid metabolism and modulates plant architecture and grain traits in durum wheat
- Open Access
A Rapid Hairy Root-Based Platform for CRISPR/Cas Optimization and Guide RNA Validation in Lettuce
Cultivated lettuce (Lactuca sativa L.) is a major leafy crop and an emerging model for functional genomics within the Asteraceae family, supported by high-quality reference genomes and efficient transformation systems. Although CRISPR/Cas technology offers powerful opportunities for crop improvement, editing efficiency depends on optimized construct architecture and reliable guide RNA (gRNA) validation. However, a rapid platform for evaluating CRISPR reagents in lettuce is still lacking. Here, we developed an efficient hairyroot-based system to accelerate CRISPR/Cas genome editing optimization in L. sativa. Four Agrobacterium rhizogenes strains were compared for hairy root induction in two cultivars, ‘Saladin’ and ‘Osiride’, identifying strain ATCC15834 as the most effective based on transformation frequency and root production. Using this platform, we evaluated multiple CRISPR construct configurations, including alternative promoters for nuclease and gRNA expression. A plant-derived promoter combined with At-pU6-26 variant significantly improved editing efficiency. As a proof of concept, we targeted LsHB2, the putative ortholog of Arabidopsis thaliana ATHB2, a key regulator of the shade avoidance response using SpCas9, SaCas9, and LbCas12a nucleases. The system enabled rapid genotyping and quantitative indel profiling. Overall, this workflow provides a robust framework for efficient guide selection and construct optimization in lettuce genome editing.
Open AccessA Rapid Hairy Root-Based Platform for CRISPR/Cas Optimization and Guide RNA Validation in Lettuce
Cultivated lettuce (Lactuca sativa L.) is a major leafy crop and an emerging model for functional genomics within the Asteraceae family, supported by high-quality reference genomes and efficient transformation systems. Although CRISPR/Cas technology offers powerful opportunities for crop improvement, editing efficiency depends on optimized construct architecture and reliable guide RNA (gRNA) validation. However, a rapid platform for evaluating CRISPR reagents in lettuce is still lacking. Here, we developed an efficient hairyroot-based system to accelerate CRISPR/Cas genome editing optimization in L. sativa. Four Agrobacterium rhizogenes strains were compared for hairy root induction in two cultivars, ‘Saladin’ and ‘Osiride’, identifying strain ATCC15834 as the most effective based on transformation frequency and root production. Using this platform, we evaluated multiple CRISPR construct configurations, including alternative promoters for nuclease and gRNA expression. A plant-derived promoter combined with At-pU6-26 variant significantly improved editing efficiency. As a proof of concept, we targeted LsHB2, the putative ortholog of Arabidopsis thaliana ATHB2, a key regulator of the shade avoidance response using SpCas9, SaCas9, and LbCas12a nucleases. The system enabled rapid genotyping and quantitative indel profiling. Overall, this workflow provides a robust framework for efficient guide selection and construct optimization in lettuce genome editing.