Alexander Klimov

The publication is devoted to the Dopplerographic examination of the renal arteries in pregnant women with chronic kidney disease (CKD) of various stages. An overview of data on the arterial renal bed and a technique for obtaining ultrasound imaging and Dopplerographic measurement of the interlobar arteries of the kidneys are presented, since it is most informative to evaluate the peak systolic velocity (PSV) in the arteries of the renal parenchyma (namely, in the interlobar arteries) for the diagnosis of renal dysfunction already at the initial stages of CKD. The diagnostic significance of the Dopplerographic parameter of peak systolic velocity (PSV) of the interlobar arteries for determining the presence of CKD and chronic renal failure (CRF) in pregnant women by trimester is highlighted. Dopplerography with assessment of the PSV of the interlobar arteries of the kidneys can be used as a non-invasive, affordable, reproducible and inexpensive screening method for CKD in the gestational period.

Background: There is growing evidence that uterine microbiota might be linked to endometrial receptivity (ER) and affect the outcome of assisted reproductive technology (ART) procedures. Owing to the invasive nature of endometrial sampling, the evaluation of microbiota in this biomaterial is only possible outside the embryo transfer (ET) cycle. However, menstrual blood might be the key to overcoming this challenge as it can be safely aspirated from the uterine cavity at the beginning of the target ET cycle. This study aimed to evaluate the association of the microbiological profiles of menstrual blood with ER in patients undergoing frozen ET. Methods: Menstrual blood was obtained from 98 individuals scheduled for frozen ET in a private ART clinic (ET success rate–50%). DNA was isolated from menstrual sediment and analyzed using a multiplex quantitative PCR assay designed to identify 28 relevant microbial taxa and 3 Herpesviridae viruses. Results: Bacterial DNA was detected in 75.5% of samples. There were no associations between the abundance of individual microbial taxa and the outcome of ET, and the same was true for Shannon’s α-diversity indices (p > 0.05). However, Candida spp. and Enterobacteriaceae were detected exclusively in patients with negative ET outcomes (p = 0.028). Individuals with recurrent implantation failure had a significantly lower abundance of Lactobacillus spp. than the rest (0.0 [0.0; 7.4] vs. 2.8 [0.0; 91.9] %, p = 0.024). Conclusions: Menstrual blood aspirated directly from the uterus is a promising biomaterial for endometrial microbiological profiling. Upon further investigation, its analysis might become a useful tool in managing infertile patients scheduled for ART treatment.