Ayşe Kanıcı Tarhane
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Ayşe Kanıcı Tarhane has a total of 7 co-authors. Here are the top co-authors by number of shared publications:
- Celal Tuğrul Zeyrek
Institute of Nuclear Sciences, Ankara University, Beşevler 10. Yıl Campus, Beşevler, Ankara 06100, Türkiye
1 shared publication - Fatih Büyük
Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University, Kars 36300, Türkiye
1 shared publication - Ibrahim Filazi
Department of Chemistry, Faculty of Arts and Sciences, University of Hatay Mustafa Kemal, Hatay, Türkiye
1 shared publication - Lütfiye Sirka
Department of Medical Services and Techniques, Şabanözü Vocational School, Çankırı Karatekin University, Çankırı 18100, Türkiye
1 shared publication - Murat Sert
Assistant Professor, Department of Medical Laboratory Techniques, Ankara Yildirim Beyazit University, Ankara, Turkey
1 shared publication - Serdal Tarhane
Veterinary Department, Sabanozu Vocational School, Çankırı Karatekin University, Çankırı, Türkiye
1 shared publication
Latest Publications
- Open Access
- 4 June 2026
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 27 (11) , 5074
Antibacterial Activity and Molecular Docking Studies of Black Cumin (Nigella sativa L.) Oil and Its Comparison with Some Antibiotics
Black cumin (Nigella sativa L.) oil has been traditionally used to manage infectious diseases. The scientific validation of its antibacterial potential remains of significant pharmacological interest. This study evaluated the in vitro antibacterial activity of cold-pressed black cumin oil against selected bacterial strains and compared its efficacy with that of common β-lactam antibiotics, supplemented by mechanistic insight through molecular docking. Pure oil was obtained via cold-press extraction from seeds. Antibacterial activity was evaluated using the disk diffusion and broth microdilution methods against Staphylococcus aureus NCTC10788, Bacillus cereus NCTC7464, Listeria monocytogenes ATCC11994, Escherichia coli NCTC2001, and Salmonella typhimurium NCTC11994. Commercial antibiotic disks containing cloxacillin (5 µg), cefoperazone (75 µg), penicillin (40 µg), and amoxicillin (25 µg) served as a reference. Potential molecular interactions were explored by the density functional theory (DFT) optimization of thymoquinone at the B3LYP/6-31+G(d,p) level, followed by molecular docking against bacterial targets. Inhibition zone diameters ranged from 13.5 ± 0.7 mm to 34 ± 2.1 mm, and minimum inhibitory concentration (MIC) values varied between 6.7 ± 2.3 and 64 ± 0.0 µg/mL depending on the bacterial strain tested. Black cumin oil demonstrated a stronger inhibitory effect on B. cereus and L. monocytogenes than the other bacteria tested, and exhibited a significantly higher inhibitory effect than some of the antibiotics tested (p < 0.05). In contrast, no statistically significant differences were observed among treatments against E. coli (p > 0.05). Overall, Gram-positive bacteria showed greater susceptibility to black cumin oil than Gram-negative bacteria. The computational analyses demonstrated stable binding interactions supporting the experimental results. These integrative in vitro and in silico findings provide mechanistic evidence for the traditional use of black cumin oil in treating infections. The results suggest that black cumin oil could be a promising natural antibacterial candidate; however, further toxicological and pharmacokinetic evaluations are required prior to clinical use.
- 4 June 2026
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 27 (11) , 5074
Open AccessAntibacterial Activity and Molecular Docking Studies of Black Cumin (Nigella sativa L.) Oil and Its Comparison with Some Antibiotics
Black cumin (Nigella sativa L.) oil has been traditionally used to manage infectious diseases. The scientific validation of its antibacterial potential remains of significant pharmacological interest. This study evaluated the in vitro antibacterial activity of cold-pressed black cumin oil against selected bacterial strains and compared its efficacy with that of common β-lactam antibiotics, supplemented by mechanistic insight through molecular docking. Pure oil was obtained via cold-press extraction from seeds. Antibacterial activity was evaluated using the disk diffusion and broth microdilution methods against Staphylococcus aureus NCTC10788, Bacillus cereus NCTC7464, Listeria monocytogenes ATCC11994, Escherichia coli NCTC2001, and Salmonella typhimurium NCTC11994. Commercial antibiotic disks containing cloxacillin (5 µg), cefoperazone (75 µg), penicillin (40 µg), and amoxicillin (25 µg) served as a reference. Potential molecular interactions were explored by the density functional theory (DFT) optimization of thymoquinone at the B3LYP/6-31+G(d,p) level, followed by molecular docking against bacterial targets. Inhibition zone diameters ranged from 13.5 ± 0.7 mm to 34 ± 2.1 mm, and minimum inhibitory concentration (MIC) values varied between 6.7 ± 2.3 and 64 ± 0.0 µg/mL depending on the bacterial strain tested. Black cumin oil demonstrated a stronger inhibitory effect on B. cereus and L. monocytogenes than the other bacteria tested, and exhibited a significantly higher inhibitory effect than some of the antibiotics tested (p < 0.05). In contrast, no statistically significant differences were observed among treatments against E. coli (p > 0.05). Overall, Gram-positive bacteria showed greater susceptibility to black cumin oil than Gram-negative bacteria. The computational analyses demonstrated stable binding interactions supporting the experimental results. These integrative in vitro and in silico findings provide mechanistic evidence for the traditional use of black cumin oil in treating infections. The results suggest that black cumin oil could be a promising natural antibacterial candidate; however, further toxicological and pharmacokinetic evaluations are required prior to clinical use.