Miroslava Strandzheva

In this paper, we describe and verify the structures and biological activity of novel metal complexes derived from 5-carboxy-2-thiouracil. We outline a synthesis method and assess the antimicrobial activity of newly formed Cu(II), Ni(II) and Co(II) complexes. Several syntheses were carried out with and without NaOH. The structures were verified using melting point analysis, MP-AES for Cu, Ni and Co and ICP-OES for sulfur, UV-Vis, ATR, solid-state and solution ¹H NMR, ¹³C NMR spectroscopy. In addition, the antimicrobial activity of these complexes and the free ligand were assessed against both Gram-positive and Gram-negative bacteria, as well as yeasts. The HCT-116 colorectal cell line (ATCC) was used for a cytotoxic assay. Only two Ni(II) complexes showed reduction in the number of viable tumor cells. None of the other compounds led to a significant reduction in the number of viable cells, as determined by one-way ANOVA statistical analysis.

The aim of this paper is to obtain and verify the structures and the biological activity of novel metal complexes derived from 5-carboxy-2-thiouracil. The paper outlines a synthesis method and assesses the antimicrobial activity of newly formed Cu(II), Ni(II) and Co(II) complexes. Several syntheses were carried out with and without NaOH. The structural were verified by melting point analysis, ICP-OES for Cu, Ni and Co, UV-Vis, ATR, solid state and solution 1H NMR, 13C NMR spectroscopy. In addition, the antimi-crobial activity of these complexes and the free ligand are assessed against both Gram-positive and Gram-negative bacteria, as well as yeasts. For the cytotoxic assay we were used the HCT-116 colorectal cell line (ATCC). Only two Ni(II) complexes show reduction in the number of viable tumor cells. All the other compounds did not lead to a significant reduction in the number of viable cells as determined by one-way ANOVA statistical analysis.

The synthesis and systematic investigation of inorganic and organic compounds are fundamental to medicinal and pharmaceutical chemistry, particularly for developing novel therapeutic agents. Transition-metal complexes and bioactive organic compounds have shown significant potential to modulate cellular pathways, yielding anticancer, antimicrobial, and anti-inflammatory effects. This review summarizes recent advances in the synthesis, structural characterization, and therapeutic evaluation of gold (Au), platinum (Pt), and ruthenium (Ru) complexes, as well as selected organic compounds, with an emphasis on their biological activities and potential clinical relevance. The integration of synthetic chemistry with biological evaluation underscores the therapeutic potential of gold (Au), platinum (Pt), and ruthenium (Ru) complexes, as well as organic compounds. Au(I/III), Pt(II/IV), and Ru(II/III) complexes demonstrated notable in vivo anticancer activity, while selected organic derivatives exhibited promising bioactive properties. This review aims to support researchers and clinicians in the ongoing development of novel compounds with clinical relevance for anticancer, antimicrobial, and anti-inflammatory applications.