Takwa Chouki
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Takwa Chouki has a total of 4 co-authors. Here are the top co-authors by number of shared publications:
- Anne Muschter
Division of Life Science & Bioprocesses, Fraunhofer Institute for Applied Polymer Research IAP, Geiselbergstr. 69, 14476 Potsdam, Germany
1 shared publication - Ruben R. Rosencrantz
Fraunhofer Institute for Applied Polymer Research IAP Life Science & Bioprocesses Geiselbergstraße 69 14476 Potsdam-Golm Germany
1 shared publication - Sophia Rosencrantz
Division of Life Science & Bioprocesses, Fraunhofer Institute for Applied Polymer Research IAP, Geiselbergstr. 69, 14476 Potsdam, Germany
1 shared publication - Stefan Reinicke
Fraunhofer Institute for Applied Polymer Research
1 shared publication
Latest Publications
- Open Access
Directed Immobilization of Horseradish Peroxidase Using a Covalently Attached Competitive Inhibitor
We developed a site-directed immobilization strategy exploiting the competitive inhibitor Remazol Brilliant Blue R (RB) to orient horseradish peroxidase (HRP) on functionalized glass surfaces. RB was covalently bound to poly(ethylene-alt-maleic anhydride) (PEMA)-coated substrates, enabling HRP binding via its active site. Subsequent contact with a second PEMA-functionalized surface facilitated covalent immobilization of HRP with controlled orientation. Interfacial layer assembly and the comparative random control were characterized by AFM and XPS analyses, while successful RB acetylation was confirmed by NMR and ATR-IR spectroscopy. Directed contact approaches using stepwise RB and PEMA+RB functionalization yielded HRP loadings with specific activities approximately twofold higher than non-directed immobilization. Our inhibitor-mediated method preserves the native enzyme structure without genetic modification, offering a straightforward platform for enhancing enzyme performance. This strategy is promising for applications in biosensors, biocatalysis, and enzyme-based devices requiring efficient enzyme immobilization.
Open AccessDirected Immobilization of Horseradish Peroxidase Using a Covalently Attached Competitive Inhibitor
We developed a site-directed immobilization strategy exploiting the competitive inhibitor Remazol Brilliant Blue R (RB) to orient horseradish peroxidase (HRP) on functionalized glass surfaces. RB was covalently bound to poly(ethylene-alt-maleic anhydride) (PEMA)-coated substrates, enabling HRP binding via its active site. Subsequent contact with a second PEMA-functionalized surface facilitated covalent immobilization of HRP with controlled orientation. Interfacial layer assembly and the comparative random control were characterized by AFM and XPS analyses, while successful RB acetylation was confirmed by NMR and ATR-IR spectroscopy. Directed contact approaches using stepwise RB and PEMA+RB functionalization yielded HRP loadings with specific activities approximately twofold higher than non-directed immobilization. Our inhibitor-mediated method preserves the native enzyme structure without genetic modification, offering a straightforward platform for enhancing enzyme performance. This strategy is promising for applications in biosensors, biocatalysis, and enzyme-based devices requiring efficient enzyme immobilization.