Andrew A. Bosah
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Interests
Andrew A. Bosah has a total of 15 co-authors. Here are the top co-authors by number of shared publications:
- Amira S. Abuhmoud
Biology Department, University of Detroit Mercy, Detroit, MI 48221, USA
1 shared publication - Antonillamarein E. Hanna
Biology Department, University of Detroit Mercy, Detroit, MI 48221, USA
1 shared publication - Brandon T. Kenaya
Biology Department, University of Detroit Mercy, Detroit, MI 48221, USA
1 shared publication - Evelyn M. Rihacek
Department of Chemistry and Biochemistry, University of Detroit Mercy, Detroit, MI 48221, USA
1 shared publication - Gregory M. Grabowski
Biology Department, University of Detroit Mercy, Detroit, MI 48221, USA
1 shared publication - Kendra R. Evans
Department of Chemistry and Biochemistry, University of Detroit Mercy, Detroit, MI 48221, USA
1 shared publication
Latest Publications
- Open Access
Exposure to the Environmental Toxins Microcystin-LR and Roundup® Causes Cellular Damage in the Hepatopancreas of the White River Crayfish (Procambarus acutus)
Aquatic ecosystems are routinely exposed to natural and anthropogenic stressors, and when multiple pollutants co-occur, their synergistic effects may exceed those of individual contaminants. This study evaluated the individual and combined effects of the widely used agrochemical Roundup®, whose active ingredient is glyphosate (GLY) and the cyanobacterial toxin microcystin-LR (MC-LR) on the hepatopancreas of crayfish. Following a seven-day exposure to MC-LR (15 ppb), GLY (75 ppb), or their combination (MC-LR + GLY), cellular and morphological alterations were assessed using flow cytometry and histological analyses. MC-LR exposure increased hepatopancreatic vacuole number 3.7-fold and vacuole proportion 6.8-fold relative to controls, whereas GLY increased vacuole proportion 5.2-fold. Combined exposure increased tubule lumen proportion by approximately 65%, while epithelial height remained unchanged. GLY reduced viable (fluorescein diacetate-positive) hepatopancreatic cells by approximately 86%, and both GLY and MC-LR + GLY increased non-viable (propidium iodide-positive) cells by 4.8-fold and 4.3-fold, respectively. MC-LR alone did not significantly affect cell viability. These findings indicate that MC-LR primarily induces structural alterations, whereas GLY causes pronounced cellular cytotoxicity. Although co-exposure did not consistently produce synergistic toxicity, it enhanced tissue remodeling, emphasizing the importance of evaluating environmentally relevant contaminant mixtures and their implications for freshwater ecosystem health.
Open AccessExposure to the Environmental Toxins Microcystin-LR and Roundup® Causes Cellular Damage in the Hepatopancreas of the White River Crayfish (Procambarus acutus)
Aquatic ecosystems are routinely exposed to natural and anthropogenic stressors, and when multiple pollutants co-occur, their synergistic effects may exceed those of individual contaminants. This study evaluated the individual and combined effects of the widely used agrochemical Roundup®, whose active ingredient is glyphosate (GLY) and the cyanobacterial toxin microcystin-LR (MC-LR) on the hepatopancreas of crayfish. Following a seven-day exposure to MC-LR (15 ppb), GLY (75 ppb), or their combination (MC-LR + GLY), cellular and morphological alterations were assessed using flow cytometry and histological analyses. MC-LR exposure increased hepatopancreatic vacuole number 3.7-fold and vacuole proportion 6.8-fold relative to controls, whereas GLY increased vacuole proportion 5.2-fold. Combined exposure increased tubule lumen proportion by approximately 65%, while epithelial height remained unchanged. GLY reduced viable (fluorescein diacetate-positive) hepatopancreatic cells by approximately 86%, and both GLY and MC-LR + GLY increased non-viable (propidium iodide-positive) cells by 4.8-fold and 4.3-fold, respectively. MC-LR alone did not significantly affect cell viability. These findings indicate that MC-LR primarily induces structural alterations, whereas GLY causes pronounced cellular cytotoxicity. Although co-exposure did not consistently produce synergistic toxicity, it enhanced tissue remodeling, emphasizing the importance of evaluating environmentally relevant contaminant mixtures and their implications for freshwater ecosystem health.