Yang Xiao
Yang Xiao has a total of 7 co-authors. Here are the top co-authors by number of shared publications:
- Hu Liu
Center for Animal Experiment/ABSL-3 Laboratory, Wuhan University, Wuhan 430071, China
1 shared publication - Jingyi Zhang
Center for Animal Experiment/ABSL-3 Laboratory, Wuhan University, Wuhan 430071, China
1 shared publication - Lianxiang Guo
Suzhou Xishan Biotech Inc., Suzhou 215021, China
1 shared publication - Rong Bao
Center for Animal Experiment/ABSL-3 Laboratory, Wuhan University, Wuhan 430071, China
1 shared publication - Yaoming Li
Applied Biotechnology Research Center, Wuhan University of Bioengineering, Wuhan 430415, China
1 shared publication - Zhang Zhang
Center for Animal Experiment/ABSL-3 Laboratory, Wuhan University, Wuhan 430071, China
1 shared publication
Latest Publications
- Open Access
- 12 September 2026
- Published by MDPI AG in Veterinary Sciences
- Vol. 13 (9) , 951
Development and Proof-of-Concept Analytical Validation of a HotStart Loop-Mediated Isothermal Amplification (LAMP) Assay Targeting the UL1 Gene for Macacine alphaherpesvirus 1 (McHV-1)
Macacine alphaherpesvirus 1 (McHV-1, commonly known as Monkey B Virus) is a fatal zoonotic pathogen naturally prevalent in macaques. In this study, we developed and conducted a proof-of-concept analytical validation of a HotStart Loop-Mediated Isothermal Amplification (LAMP) assay targeting a conserved, moderately GC-rich (61–62%) region of the UL1 gene in McHV-1. The reaction was optimized at 70 °C using engineered HotStart Bst 3.2/4.2 DNA Polymerase, tracked via real-time fluorescence and closed-tube visual colorimetric detection (pre-added L-HNB dye) without post-amplification tube opening to reduce the risk of post-amplification aerosol carry-over contamination. The assay achieved a preliminary practical analytical limit of detection (analytical LOD) of 100 copies/reaction (3/3 replicates) within a 15–30 min diagnostic cutoff window, matching the practical sensitivity of reference qPCR. Strict analytical specificity was demonstrated against a comprehensive panel of human herpesviruses (HHV-1 through HHV-5) and host macaque genomic DNA. As a single-target analytical prototype, this visual HotStart LAMP platform provides a rapid, equipment-free molecular tool with promising potential for McHV-1 surveillance in resource-limited settings and macaque breeding facilities upon further clinical validation.
- 12 September 2026
- Published by MDPI AG in Veterinary Sciences
- Vol. 13 (9) , 951
Open AccessDevelopment and Proof-of-Concept Analytical Validation of a HotStart Loop-Mediated Isothermal Amplification (LAMP) Assay Targeting the UL1 Gene for Macacine alphaherpesvirus 1 (McHV-1)
Macacine alphaherpesvirus 1 (McHV-1, commonly known as Monkey B Virus) is a fatal zoonotic pathogen naturally prevalent in macaques. In this study, we developed and conducted a proof-of-concept analytical validation of a HotStart Loop-Mediated Isothermal Amplification (LAMP) assay targeting a conserved, moderately GC-rich (61–62%) region of the UL1 gene in McHV-1. The reaction was optimized at 70 °C using engineered HotStart Bst 3.2/4.2 DNA Polymerase, tracked via real-time fluorescence and closed-tube visual colorimetric detection (pre-added L-HNB dye) without post-amplification tube opening to reduce the risk of post-amplification aerosol carry-over contamination. The assay achieved a preliminary practical analytical limit of detection (analytical LOD) of 100 copies/reaction (3/3 replicates) within a 15–30 min diagnostic cutoff window, matching the practical sensitivity of reference qPCR. Strict analytical specificity was demonstrated against a comprehensive panel of human herpesviruses (HHV-1 through HHV-5) and host macaque genomic DNA. As a single-target analytical prototype, this visual HotStart LAMP platform provides a rapid, equipment-free molecular tool with promising potential for McHV-1 surveillance in resource-limited settings and macaque breeding facilities upon further clinical validation.