Jingjing Yan
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Jingjing Yan has a total of 7 co-authors. Here are the top co-authors by number of shared publications:
- Gaohan Yue
College of Agriculture and Animal Husbandry Qinghai University, Xining, China, China
1 shared publication - Guoxiu Li
College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
1 shared publication - Haoyuan Wang
Livestock and Veterinary Workstation in Changmu Town, Livestock and Veterinary Workstation in Changmu Town, Xining, China, China
1 shared publication - Jianshu Lv
College of Agriculture and Animal Husbandry Qinghai University, Xining, China, China
1 shared publication - Kuangyan Li
College of Agriculture and Animal Husbandry Qinghai University, Xining, China, China
1 shared publication - Shiqi Chen
College of Agriculture and Animal Husbandry Qinghai University, Xining, China, China
1 shared publication
Latest Publications
- Open Access
- 8 October 2026
- Published by MDPI AG in Preprints.org
MT1 Mediated Melatonin Attenuates High-Dose DHT Induced Premature Development of Secondary Follicles in the Ovine Ovary In Vitro
Premature ovarian failure, resulting from the premature activation of follicles in animals, has been linked to excessive levels of 5α-dihydrotestosterone (DHT). Additionally, melatonin has been demonstrated to be autonomously synthesized and secreted by sheep follicles. However, it remains unclear whether melatonin can counteract excessive DHT in sheep ovaries to support normal follicular development. Here, we investigated the effects of DHT and melatonin on early folliculogenesis in ovine ovaries. Ovarian cortices (~2×2 mm) and secondary follicles (280–360 μm) were cultured for 4 days with high-dose DHT (10⁻⁷ M) and/or melatonin (10⁻⁹ M). H&E staining, TUNEL assay, Western blotting, and RT-qPCR were used to examine the underlying mechanisms. DHT increased the number and diameter of secondary follicles (p < 0.05), but also induced granulosa cell apoptosis, which melatonin alleviated. Mechanistically, DHT upregulated cleaved caspase-3 and caspase-3 expression in granulosa cells, whereas co-treatment with melatonin reduced the Bcl-2/Bax ratio. Using the MT1 inhibitor Luzindole, melatonin was shown to suppress DHT-stimulated granulosa cell proliferation via MT1-mediated Akt phosphorylation, thereby counteracting the DHT-induced decline in follicular reserve. This study elucidates the pathogenic mechanism of DHT-triggered premature ovarian insufficiency and validates the melatonin-mediated protective regulation of follicular development, which provides a theoretical basis for the improvement and protection of reproductive function in reserve breeding ewes.
- 8 October 2026
- Published by MDPI AG in Preprints.org
Open AccessMT1 Mediated Melatonin Attenuates High-Dose DHT Induced Premature Development of Secondary Follicles in the Ovine Ovary In Vitro
Premature ovarian failure, resulting from the premature activation of follicles in animals, has been linked to excessive levels of 5α-dihydrotestosterone (DHT). Additionally, melatonin has been demonstrated to be autonomously synthesized and secreted by sheep follicles. However, it remains unclear whether melatonin can counteract excessive DHT in sheep ovaries to support normal follicular development. Here, we investigated the effects of DHT and melatonin on early folliculogenesis in ovine ovaries. Ovarian cortices (~2×2 mm) and secondary follicles (280–360 μm) were cultured for 4 days with high-dose DHT (10⁻⁷ M) and/or melatonin (10⁻⁹ M). H&E staining, TUNEL assay, Western blotting, and RT-qPCR were used to examine the underlying mechanisms. DHT increased the number and diameter of secondary follicles (p < 0.05), but also induced granulosa cell apoptosis, which melatonin alleviated. Mechanistically, DHT upregulated cleaved caspase-3 and caspase-3 expression in granulosa cells, whereas co-treatment with melatonin reduced the Bcl-2/Bax ratio. Using the MT1 inhibitor Luzindole, melatonin was shown to suppress DHT-stimulated granulosa cell proliferation via MT1-mediated Akt phosphorylation, thereby counteracting the DHT-induced decline in follicular reserve. This study elucidates the pathogenic mechanism of DHT-triggered premature ovarian insufficiency and validates the melatonin-mediated protective regulation of follicular development, which provides a theoretical basis for the improvement and protection of reproductive function in reserve breeding ewes.