Determination of trans‐fatty acids in food samples based on the precolumn fluorescence derivatization by high performance liquid chromatography

Authors:
Publisher website
Google Scholar
Abstract
Trans‐fatty acids are unsaturated fatty acids that are considered to have health risks. 1,3,5,7‐Tetramethyl‐8‐butyrethylenediamine‐difluoroboradiaza‐s‐indacene is a highly sensitive fluorescent labeling reagent for carboxylic acids developed by our lab. In this study, using this precolumn fluorescent derivatization reagent, a rapid and accurate high‐performance liquid chromatography with fluorescence detection method was developed for the determination of two trans‐fatty acids in food samples. Under the optimized derivative conditions, two trans‐fatty acids were tagged with the fluorescent labeling reagent in the presence of 1‐ethyl‐3‐(3‐dimethyl‐aminopropyl) carbodiimide at 25°C for 30 min. Then, the baseline separation of trans‐ and cis‐fatty acids and their saturated fatty acid with similar structures was achieved with less interference using a reversed‐phased C18 column with isocratic elution in 14 min. With fluorescence detection at λex/λem = 490 /510 nm, the linear range of the TFAs was 1.0‐200 nM with low detection limits in the range of 0.1–0.2 nM (signal‐to‐noise ratio = 3). In addition, the proposed approach was successfully applied for the detection of trans‐fatty acids in food samples, and the recoveries using this method ranged from 96.02 to 109.22% with low relative standard deviations of 1.2–4.3% (n = 6).
Funding Information
  • National Natural Science Foundation of China (51578538, 31670370)
  • Major Science and Technology Program for Water Pollution Control and Treatment (2017ZX07205‐002‐R08)

This publication has 39 references indexed in Scilit: