Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer.
Authors:
- W M YokoyamaWYW M Yokoyama
- F KoningFKF Koning
- G StinglGSG Stingl
- J A BluestoneJBJ A Bluestone
- J E ColiganJCJ E Coligan
- E M ShevachESE M Shevach
Open Access
- 1 June 1987
- journal article
- research article
- Published by Rockefeller University Press in Journal of Experimental Medicine
- Vol. 165 (6) , 1725-1730
Abstract
We have produced a T cell hybridoma line by fusion of an IL-2-dependent, long-term T cell receptor (TCR) gamma/delta+ Thy-1+, bone marrow-derived, dendritic epidermal cell line to the BW5147 tumor line. The resultant hybridoma was rapidly growing, lymphokine independent, and expressed T3 in association with the TCR gamma/delta heterodimer. Several subclones of the hybridoma line produced easily detectable levels of IL-2 after stimulation by anti-T3 or Con A. The availability of these cloned cell lines should greatly facilitate further functional, biochemical, and molecular studies of the TCR delta chain.Keywords
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