Dynamic Epigenetic Changes Involved in Testicular Toxicity Induced by Di‐2‐(Ethylhexyl) Phthalate in Mice
Authors:
Open Access
- 11 January 2010
- journal article
- Published by Wiley in Basic & Clinical Pharmacology & Toxicology
- Vol. 106 (2) , 118-123
Abstract
Abstract: The aim of this study was to analyse epigenetic (specifically, DNA methylation) change in testes induced by maternal exposure to di‐2‐(ethylhexyl) phthalate (DEHP) in mice. Testicular dysgenesis syndrome was induced in foetuses by maternal exposure to DEHP. High‐performance liquid chromatography was performed to analyse DNA methylation status, and expression levels of the DNA methyltransferases were examined by quantitative real‐time polymerase chain reaction and western blotting. DEHP significantly had more than 10% relative increase in the global DNA methylation and also increased DNA methyltransferases’ expression. Changes in DNA methylation may play an important role in abnormal testicular function caused by environmental factors such as maternal exposure to DEHP, which may be one possible mechanism of DEHP‐mediated testicular toxicity.Keywords
This publication has 53 references indexed in Scilit:
- Mechanisms of peroxisome proliferator-induced DNA hypomethylation in rat liverMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2008
- Developmental origins of disease and determinants of chromatin structure: maternal diet modifies the primate fetal epigenomeJournal of Molecular Endocrinology, 2008
- How Are Alkynes Scrambled?Science, 2005
- Murine De Novo Methyltransferase Dnmt3a Demonstrates Strand Asymmetry and Site Preference in the Methylation of DNA In VitroMolecular and Cellular Biology, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Enzymatic properties of recombinant Dnmt3a DNA methyltransferase from mouse: the enzyme modifies DNA in a non-processive manner and also methylates non-CpA sitesJournal of Molecular Biology, 2001
- Role for DNA methylation in genomic imprintingNature, 1993
- Activation of immediate‐early gene expression by peroxisome proliferators in vitroMolecular Carcinogenesis, 1993
- Targeted mutation of the DNA methyltransferase gene results in embryonic lethalityCell, 1992
- MECHANISMS OF X-CHROMOSOME REGULATIONAnnual Review of Genetics, 1988