Identification of chemosensory genes from the antennal transcriptome ofSemiothisa cinerearia
Authors:
Open Access
- 7 August 2020
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 15 (8) , e0237134
Abstract
Olfaction plays vital roles in the survival and reproduction of insects. The completion of olfactory recognition requires the participation of various complex protein families. However, little is known about the olfactory-related proteins inSemiothisa cinereariaBremer et Grey, an important pest of Chinese scholar tree. In this study, we sequenced the antennal transcriptome ofS.cinereariaand identified 125 olfactory-related genes, including 25 odorant-binding proteins (OBPs), 15 chemosensory proteins (CSPs), two sensory neuron membrane proteins (SNMPs), 52 odorant receptors (ORs), eight gustatory receptors (GRs) and 23 ionotropic receptors (IRs). BLASTX best hit results and phylogenetic analyses indicated that these genes were most identical to their respective orthologs fromEctropis obliqua. Further quantitative real-time PCR (qRT-PCR) analysis revealed that three ScinOBPs and three ScinORs were highly expressed in male antennae, while seven ScinOBPs and twelve ScinORs were female-specifically expressed. Our study will be useful for the elucidation of olfactory mechanisms inS.cinerearia.Keywords
Funding Information
- HAAFS Agriculture Science and Technology Innovation Project (2019-1-2-2)
- Hebei Province Research and Development Program (20326518D)
- HAAFS Research and Development Program (2018120304)
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