Determination of herpes simplex virus type-specific antibodies by enzyme-linked immunosorbent assay
Authors:
- R M ColemanRCR M Coleman
- L PereiraLPL Pereira
- P D BaileyPBP D Bailey
- D DonderoDDD Dondero
- C WickliffeCWC Wickliffe
- A J NahmiasANA J Nahmias
- 1 August 1983
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 18 (2) , 287-291
Abstract
We determined type-specific antibodies to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) by an indirect enzyme-linked immunosorbent assay, using as antigens HSV-1 glycoprotein gC-1 and a HSV-2-specific polypeptide purified on affinity columns of monoclonal antibodies. All sera were initially screened for HSV antibodies by the enzyme-linked immunosorbent assay with a pool of Triton X-100-extracted antigens of HSV-1- and HSV-2-infected HEp-2 cells. The titer of HSV antibodies was predicted from a linear regression curve based on the absorbance of the initial 1:50 serum dilution. The sensitivity and specificity of the screening assay and of the assay for type-specific antibodies were established.Keywords
This publication has 26 references indexed in Scilit:
- Elisa for Herpes Simplex Virus (HSV) Type‐Specific Antibodies in Human Sera Using HSV Type 1 and Type 2 Polyspecific Antigens Blocked with Type‐Heterologous Rabbit AntibodiesActa Pathologica Microbiologica Scandinavica Section B Microbiology, 1979
- Detection of Herpesvirus Type-Specific Antibody by a Micro Solid Phase Radioimmunometric AssayIntervirology, 1979
- Antibodies to herpes simplex virus types 1 and 2 in patients with squamous‐cell carcinoma of uterine cervix in IndiaInternational Journal of Cancer, 1978
- Differentiation of Serum Antibodies to Herpesvirus Types 1 and 2 by RadioimmunoassayExperimental Biology and Medicine, 1978
- TITRATION OF HERPES SIMPLEX VIRUS ANTIBODIES IN HUMAN SERA BY THE ENZYME‐LINKED IMMUNOSORBENT ASSAY (ELISA)Acta Pathologica Microbiologica Scandinavica Section B Microbiology, 1977
- Effect of Cross-Immunization on Monotypic Antibody Responses to Herpes Simplex Virus Types 1 and 2The Journal of Immunology, 1976
- Antibody-Dependent Cell-Mediated Cytotoxicity to Target Cells Infected with Type 1 and Type 2 Herpes Simplex VirusThe Journal of Immunology, 1976
- Typing Herpesvirus hominis antibodies and isolates by inhibition of the indirect hemagglutination reaction.1974
- Method for typing antisera to Herpesvirus hominis by indirect hemagglutination inhibition.1971
- Measurement of Antibodies to Herpesvirus Types 1 and 2 in Human SeraThe Journal of Immunology, 1970