Enhancement of Autophagy in Macrophages via the p120-Catenin-Mediated mTOR Signaling Pathway

Authors:
Publisher website
Google Scholar
Abstract
Autophagy serves as a critical regulator of immune responses in sepsis. Macrophages are vital constituents of both innate and adaptive immunity. In this study, we delved into the intricate role of p120-catenin (p120) in orchestrating autophagy in macrophages in response to endotoxin stimulation. Depletion of p120 effectively suppressed LPS-induced autophagy in both J774A.1 macrophages and murine bone marrow–derived macrophages. LPS not only elevated the interaction between p120 and L chain 3 (LC3) I/II but also facilitated the association of p120 with mammalian target of rapamycin (mTOR). p120 depletion in macrophages by small interfering RNA reduced LPS-induced dissociation of mTOR and Unc-51–like kinase 1 (ULK1), leading to an increase in the phosphorylation of ULK1. p120 depletion also enhanced LPS-triggered macrophage apoptosis, as evidenced by increased levels of cleaved caspase 3, 7-aminoactinomycin D staining, and TUNEL assay. Notably, inhibiting autophagy reversed the decrease in apoptosis caused by LPS stimulation in macrophages overexpressing p120. Additionally, the ablation of p120 inhibited autophagy and accentuated apoptosis in alveolar macrophages in LPS-challenged mice. Collectively, our findings strongly suggest that p120 plays a pivotal role in fostering autophagy while concurrently hindering apoptosis in macrophages, achieved through modulation of the mTOR/ULK1 signaling pathway in sepsis. This underscores the potential of targeting macrophage p120 as an innovative therapeutic avenue for treating inflammatory disorders.
Funding Information
  • HHS | NIH | National Heart, Lung, and Blood Institute (R01HL152696)
  • HHS | NIH | National Heart, Lung, and Blood Institute (R01HL104092)
  • HHS | NIH | National Institute of Allergy and Infectious Diseases (R21AI152249)
  • HHS | NIH | National Institute of General Medical Sciences (GM117628)
  • HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (DK123826)
  • HHS | NIH | NIDDK | Division of Diabetes, Endocrinology, and Metabolic Diseases (DK129960)
  • US Department of Veterans Affairs Merit Review Award (I01BX001690)

This publication has 47 references indexed in Scilit: