DNA Sequence and Regional Assignment of the Human Follicle-Stimulating Hormone β-Subunit Gene to the Short Arm of Human Chromosome 11

Authors:
  • PWPaul C. Watkins
    Paul C. Watkins
  • RERoger Eddy
    Roger Eddy
  • ABAnton K. Beck
    Anton K. Beck
  • VVVincent Vellucci
    Vincent Vellucci
  • BLBenjamin Leverone
    Benjamin Leverone
  • RTRudolph E. Tanzi
    Rudolph E. Tanzi
  • JGJames F. Gusella
    James F. Gusella
  • TSThomas B. Shows
    Thomas B. Shows
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Abstract
A human genomic DNA fragment in phage λ containing FSHB , the gene for the β-subunit of human follicle-stimulating hormone (FSH-β), was analyzed and the nucleotide sequence of the region of the clone encoding FSH-β was determined. A subclone of the λ phage containing 67% of FSH-β coding sequence was used as hybridization probe to determine the human chromosomal location of FSHB . A panel of mouse-human somatic cell hybrids containing reduced numbers of human chromosomes was screened with the FSHB probe; complete cosegregation of FSHB with human chromosome 11 was observed in all 26 cell hybrids tested. Analysis of a set of cell hybrids containing translocated derivatives of chromosome 11 further localized FSHB to the human chromosome region llpll.2→11pter. A Hin d III restriction fragment length polymorphism (RFLP) detected by another subclone of the λ phage containing FSHB now provides a genetic marker for this region of the human genome.

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