Investigation of Antioxidant, Antidiabetic and Antimicrobial Activities of Timmia megapolitana (Bryophyta)

Authors:
Publisher website
Google Scholar
Abstract
Bryophytes are an underexplored source of natural compounds with diverse biological activities. In this study evaluated the antioxidant, antidiabetic, and antimicrobial properties of methanol and acetone extracts of Timmia megapolitana. Antioxidant activity was determined using DPPH• and ABTS•+ radical scavenging methods, while antidiabetic activity was assessed based on α-amylase inhibition. Both extracts exhibited concentration dependent antioxidant and α-amylase inhibitory activities. The acetone extract exhibited higher activity than the methanol extract against DPPH•, ABTS•+, and α-amylase, with IC₅₀ values of 794.13, 752.37, and 939.84 µg/mL, respectively. In the methanol extract, the corresponding IC₅₀ values were 888.84, 834.04, and 971.55 µg/mL, respectively. Although both extracts were less active than the reference compounds, their concentration-dependent activities demonstrated their biological potential. The antimicrobial activity of T. megapolitana extracts was assessed against ten bacterial and two yeast strains. The efficacy of the extracts was determined by disk diffusion (ZOI) and liquid microdilution (MIC) methods. The acetone extract showed high antifungal activity against C. albicans with a 25 mm inhibition zone and a MIC value of 16 µg/mL. The methanol extract showed remarkable antibacterial effect against S. typhimurium with a ZOI of 18 mm and a MIC value of 16 µg/mL. Moderate effects were observed against B. cereus and S. dysenteriae, but some standard strains such as S. aureus and E. coli were resistant. Overall, T. megapolitana demonstrated promising antimicrobial activity against C. albicans and certain Gram-negative bacteria, suggesting that it could be a potential source of bioactive compounds.
Funding Information
  • The author declares that no funding, grant, or any other form of support was received during the preparation of this manuscript.

This publication has 43 references indexed in Scilit: