Reconstitution System of NLRP3 Inflammasome in HEK293T Cells
Authors:
- 1 January 2022
- book chapter
- Published by Springer Nature
- Vol. 2459, 79-84
Abstract
NLRP3 (NOD-, LRR-, and pyrin domain-containing protein 3) inflammasome is a cytosolic multimeric protein complex that plays key roles in the host innate immune response to both pathogenic and sterile insults. Here we describe a comprehensive guide to study NLRP3 inflammasome activation in HEK293T cell reconstitution system, which could provide direct biochemical evidence in protein interaction and posttranslational modification of the complex.Keywords
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