The effects of vitamin C and methionine hydroxy analog supplementation on performance, blood parameters, liver enzymes, thyroid hormones, antioxidant activity of blood plasma, intestine morphology, and HSP70 gene expression of broilers under heat stress

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Abstract
This experiment was performed to evaluate the effect of vitamin C and methionine hydroxy analog (MHA) on performance, blood parameters, liver enzymes, activity of selected hormones, antioxidant activity of blood plasma, intestine morphology, and HSP70 gene expression in broilers under heat stress. Four hundred 1-day-old male chickens of commercial Ross 308 strain were used in a completely randomized design with 4 treatments and 5 replicates of 20 chickens each. The experimental treatments were as follows: (1) base diet based on maize soybean (the control), (2) base diet with 0.2% vitamin C, (3) base diet with 0.46, 0.36, and, 0.32% methionine hydroxy analog, in the starter, grower, and finisher periods, respectively, and (4) base diet with a mixture of 0.2% vitamin C and 0.46, 0.36, and 0.32% of methionine hydroxy analog in the starter, grower, and finisher periods respectively. Although numerically higher than control, the effect of the experimental diets on performance was not significant across the experimental period (P>0.05). Glucose and LDL concentrations under all experimental treatments were significantly reduced compared to the control (PPPPHSP70 gene expression was significantly reduced in all of the treatments compared to the control (P<0.05). The results of this experiment showed that the use of vitamin C and methionine hydroxy analog (MHA) can be effective in reducing the negative effects of heat stress.

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