Fe,Zr-MOF Nanozyme-Enhanced Dual-Recognition System: Integrating Molecularly Imprinted Polymers and Aptamers for Colorimetric Detection of Chlorpyrifos

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Abstract
Chlorpyrifos (CPF) poses severe environmental and health risks, driving the need for on-site monitoring tools. This study presents a novel dual-recognition colorimetric sensor for ultrasensitive detection of CPF by coimmobilizing molecularly imprinted polymers (MIPs) and aptamers onto bimetallic Fe,Zr-MOF composites. The resulting Fe,Zr-MOF/Apt/MIP hybrid synergistically merges the structural robustness of MIPs with the conformational specificity of aptamers, while also functioning as both a nanocarrier and a peroxidase mimic. Notably, CPF binding sterically hinders substrate diffusion to catalytic sites, enabling quantitative detection via dose-dependent inhibition of TMB oxidation. The optimized sensor exhibits exceptional performance: a broad linear range (1-120 ng mL-1), ultralow detection limit (0.105 ng mL-1), and high specificity against interferents. When validated with complex matrices (apples, pears, and cabbage), it achieved satisfactory recoveries (91.0-110.0%). This work establishes a field-deployable strategy for on-site pesticide monitoring in food and environmental safety.
Funding Information
  • Hubei Provincial Department of Education (Q20231304)
  • Central Public-interest Scientific Institution Basal Research Fund (1610012024005)

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