Transcriptomic effects of di-(2-ethylhexyl)-phthalate in Syrian hamster embryo cells: an important role of early cytoskeleton disturbances in carcinogenesis?
Open Access
- 25 October 2011
- journal article
- research article
- Published by Springer Nature in BMC Genomics
- Vol. 12 (1) , 524
Abstract
Di-(2-ethylhexyl)-phthalate (DEHP) is a commonly used plasticizer in polyvinylchloride (PVC) formulations and a potentially non-genotoxic carcinogen. The aim of this study was to identify genes whose level of expression is altered by DEHP by using a global wide-genome approach in Syrian hamster embryo (SHE) cells, a model similar to human cells regarding their responses to this type of carcinogen. With mRNA Differential Display (DD), we analysed the transcriptional regulation of SHE cells exposed to 0, 12.5, 25 and 50 μM of DEHP for 24 hrs, conditions which induced neoplastic transformation of these cells. A real-time quantitative polymerase chain reaction (qPCR) was used to confirm differential expression of genes identified by DD.Keywords
This publication has 77 references indexed in Scilit:
- Gene expression profiling of DEHP-treated cardiomyocytes reveals potential causes of phthalate arrhythmogenicityToxicology, 2011
- Coronin 1C negatively regulates cell–matrix adhesion and motility of intestinal epithelial cellsBiochemical and Biophysical Research Communications, 2010
- Characterization of Peroxisome Proliferator–Activated Receptor α—Independent Effects of PPARα Activators in the Rodent Liver: Di-(2-ethylhexyl) phthalate also Activates the Constitutive-Activated ReceptorToxicological Sciences, 2009
- Signaling, physiological functions and clinical relevance of the G protein-coupled estrogen receptor GPERProstaglandins & Other Lipid Mediators, 2009
- Expression and prognostic significance of THBS1, Cyr61 and CTGF in esophageal squamous cell carcinomaBMC Cancer, 2009
- Association of Genetic Variation in Genes Implicated in the β-Catenin Destruction Complex with Risk of Breast CancerCancer Epidemiology, Biomarkers & Prevention, 2008
- PPARα‐ and DEHP‐Induced CancersPPAR Research, 2008
- Cell adhesion and signalling by cadherins and Ig-CAMs in cancerNature Reviews Cancer, 2004
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Cortactin: coupling membrane dynamics to cortical actin assemblyOncogene, 2001