Altair-dvOPM: an open-access platform for large-field three-dimensional tissue imaging

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Abstract
Altair-dvOPM is an open-access direct-view oblique plane microscope designed for large-field, three-dimensional imaging of cleared and expanded tissue sections. By combining photographic-lens-based detection, externally launched oblique illumination and precision-registered modular baseplates, the system achieves micrometer-scale lateral resolution over a ~5.4 mm field of view without custom objectives or highly specialized alignment procedures. We demonstrate imaging across scales, from subcellular structures in expanded cells to centimeter-scale expanded tissue sections, and provide documentation, CAD files, Zemax models and open-source control software to support replication and extension.
Funding Information
  • National Cancer Institute (U54CA268072)
  • National Institute of General Medical Sciences (RM1GM145399)
  • National Institute of General Medical Sciences (R35GM133522)
  • National Institute of Biomedical Imaging and Bioengineering (R01EB035538)
  • National Institute of Neurological Disorders and Stroke (R01NS110853)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DP1DK139976)
  • National Institute on Alcohol Abuse and Alcoholism (R01AA028791)

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