Bingying Chen
Research Subjects
Bingying Chen has a total of 17 co-authors. Here are the top co-authors by number of shared publications:
- Kevin M. Dean
Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, USA
2 shared publications - Qionghua Shen
Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, USA
2 shared publications - Reto Fiolka
Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, USA
2 shared publications - Seweryn Gałecki
Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, USA
2 shared publications - Bo-Jui Chang
Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, USA
1 shared publication Daniel Stoddard
UT Southwestern Medical Center
1 shared publication
Latest Publications
- Open Access
Altair-dvOPM: an open-access platform for large-field three-dimensional tissue imaging
Altair-dvOPM is an open-access direct-view oblique plane microscope designed for large-field, three-dimensional imaging of cleared and expanded tissue sections. By combining photographic-lens-based detection, externally launched oblique illumination and precision-registered modular baseplates, the system achieves micrometer-scale lateral resolution over a ~5.4 mm field of view without custom objectives or highly specialized alignment procedures. We demonstrate imaging across scales, from subcellular structures in expanded cells to centimeter-scale expanded tissue sections, and provide documentation, CAD files, Zemax models and open-source control software to support replication and extension.
Open AccessAltair-dvOPM: an open-access platform for large-field three-dimensional tissue imaging
Altair-dvOPM is an open-access direct-view oblique plane microscope designed for large-field, three-dimensional imaging of cleared and expanded tissue sections. By combining photographic-lens-based detection, externally launched oblique illumination and precision-registered modular baseplates, the system achieves micrometer-scale lateral resolution over a ~5.4 mm field of view without custom objectives or highly specialized alignment procedures. We demonstrate imaging across scales, from subcellular structures in expanded cells to centimeter-scale expanded tissue sections, and provide documentation, CAD files, Zemax models and open-source control software to support replication and extension.
- Open Access
Unraveling Subcellular Ultrastructure with Cyclically Multiplexed Expansion Microscopy
Despite advances in fluorescence microscopy, spectral overlap and limited resolution hinder the dense mapping of the cellular ultrastructure. To overcome these challenges, we developed Cy-ExM, a high-plex imaging strategy that integrates optimized cryo-fixation for antigen preservation, expansion microscopy, and iterative immunofluorescence labeling. Using oblique plane microscopy, we perform three-dimensional super-resolution imaging of 20 biological targets encompassing the full cellular volume of individual mammalian cells.
Open AccessUnraveling Subcellular Ultrastructure with Cyclically Multiplexed Expansion Microscopy
Despite advances in fluorescence microscopy, spectral overlap and limited resolution hinder the dense mapping of the cellular ultrastructure. To overcome these challenges, we developed Cy-ExM, a high-plex imaging strategy that integrates optimized cryo-fixation for antigen preservation, expansion microscopy, and iterative immunofluorescence labeling. Using oblique plane microscopy, we perform three-dimensional super-resolution imaging of 20 biological targets encompassing the full cellular volume of individual mammalian cells.