Multilocus molecular phylogenetic-led discovery and formal recognition of four novel root-colonizing Fusarium species from northern Kazakhstan and the phylogenetically divergent Fusarium steppicola lineage

Authors:
Open Access
Publisher website
Google Scholar
Abstract
In this study, DNA sequence data were used to characterize 290 Fusarium strains isolated during a survey of root-colonizing endophytic fungi of agricultural and nonagricultural plants in northern Kazakhstan. The Fusarium collection was screened for species identity using partial translation elongation factor 1-α (TEF1) gene sequences. Altogether, 16 different Fusarium species were identified, including eight known and four novel species, as well as the discovery of the phylogenetically divergent F. steppicola lineage. Isolates of the four putatively novel fusaria were further analyzed phylogenetically with a multilocus data set comprising partial sequences of TEF1, RNA polymerase II largest (RPB1) and second-largest (RPB2) subunits, and calmodulin (CaM) to assess their genealogical exclusivity. Based on the molecular phylogenetic and comprehensive morphological analyses, four new species are formally described herein: F. campestre, F. kazakhstanicum, F. rhizicola, and F. steppicola.
Funding Information
  • National Research, Development and Innovation Office (TKP2020-IKA-05)
  • Hungarian-Chinese industrial research and development cooperation tender (2017-2.3.6-TÉT-CN-2018-00025)
  • National Research, Development and Innovation Office (OTKA KH-130401 and K-139026)
  • Agricultural Research Service (NSF DEB-1655980)
  • Oak Ridge Institute for Science and Education
  • ORISE
  • ORISE
  • ORISE

This publication has 77 references indexed in Scilit: