Expression of a chemically synthesized human alpha 1 interferon gene.
Authors:
- E De MaeyerEME De Maeyer
- D SkupDSD Skup
- K S PrasadKPK S Prasad
- J De Maeyer-GuignardJMJ De Maeyer-Guignard
- B WilliamsBWB Williams
- P MeacockPMP Meacock
- G SharpeGSG Sharpe
- D PioliDPD Pioli
- J HennamJHJ Hennam
- W SchuchWSW Schuch
- K AthertonKAK Atherton
- 1 July 1982
- journal article
- Published by National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (14) , 4256-4259
Abstract
Cells of Escherichia coli containing a chemically synthesized human alpha 1 interferon (IFN-alpha 1) gene, under control of the lac promoter, make a product with biological properties indistinguishable from those of the natural IFN-alpha 1 [antiviral activity, acid stability, species crossreactivity, inactivation by antisera directed against leukocyte or Namalwa cell interferon, and stimulation of (2'-5')oligoadenylate synthetase activity]. Similar levels of IFN synthesis were obtained when the expression unit (lac promoter plus synthetic IFN-alpha 1 gene) was transplanted into the obligate methylotroph Methylophilus methylotrophus.Keywords
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