Genome-Wide Identification of the TGA Gene Family and Expression Analysis under Drought Stress in Brassica napus L.
Open Access
- 8 June 2024
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 25 (12) , 6355
Abstract
TGA transcription factors belong to Group D of the bZIP transcription factors family and play vital roles in the stress response of plants. Brassica napus is an oil crop with rich economic value. However, a systematic analysis of TGA gene family members in B. napus has not yet been reported. In this study, we identified 39 full-length TGA genes in B. napus, renamed TGA1~TGA39. Thirty-nine BnTGA genes were distributed on 18 chromosomes, mainly located in the nucleus, and differences were observed in their 3D structures. Phylogenetic analysis showed that 39 BnTGA genes could be divided into five groups. The BnTGA genes in the same group had similar structure and motif compositions, and all the BnTGA genes had the same conserved bZIP and DOG1 domains. Phylogenetic and synteny analysis showed that the BnTGA genes had a close genetic relationship with the TGA genes of the Brassica juncea, and BnTGA11 and BnTGA29 may play an important role in evolution. In addition, qRT-PCR revealed that three genes (BnTGA14/17/23) showed significant changes in eight experimental materials after drought treatment. Meanwhile, it can be inferred from the results of drought treatment on different varieties of rapeseed that the stress tolerance of parental rapeseed can be transmitted to the offspring through hybridization. In short, these findings have promoted the understanding of the B. napus TGA gene family and will contribute to future research aimed at B. napus resistant breeding.Keywords
Funding Information
- Agriculture and Rural Affairs Department of Zhejiang Province (2023SNJF005, 2022C02034, 2022NZZJ0018, 2021YFYZ0018, 23042097-Y)
- Science and Technology Department of Zhejiang Province (2023SNJF005, 2022C02034, 2022NZZJ0018, 2021YFYZ0018, 23042097-Y)
- Science and Technology Program of Sichuan Province (2023SNJF005, 2022C02034, 2022NZZJ0018, 2021YFYZ0018, 23042097-Y)
- Zhejiang Sci-Tech University (2023SNJF005, 2022C02034, 2022NZZJ0018, 2021YFYZ0018, 23042097-Y)
- China Scholarship Council (2023SNJF005, 2022C02034, 2022NZZJ0018, 2021YFYZ0018, 23042097-Y)
This publication has 74 references indexed in Scilit:
- Tobacco bZIP transcription factor TGA2.2 and related factor TGA2.1 have distinct roles in plant defense responses and plant developmentThe Plant Journal, 2005
- Knockout Analysis of Arabidopsis Transcription Factors TGA2, TGA5, and TGA6 Reveals Their Redundant and Essential Roles in Systemic Acquired ResistanceThe Plant Cell, 2003
- Transcription factors in plant defense and stress responsesCurrent Opinion in Plant Biology, 2002
- Identification, Analysis, and Utilization of Conserved Ortholog Set Markers for Comparative Genomics in Higher PlantsThe Plant Cell, 2002
- bZIP transcription factors in ArabidopsisTrends in Plant Science, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Trans‐dominant suppression of plant TGA factors reveals their negative and positive roles in plant defense responsesThe Plant Journal, 2001
- NPR1 Differentially Interacts with Members of the TGA/OBF Family of Transcription Factors That Bind an Element of the PR-1 Gene Required for Induction by Salicylic AcidMolecular Plant-Microbe Interactions®, 2000
- Mutations in the PERIANTHIA gene of Arabidopsis specifically alter floral organ number and initiation patternDevelopment, 1996
- Two tobacco DNA-binding proteins with homology to the nuclear factor CREBNature, 1989