Direct Visualization of A-, P-, and E-Site Transfer RNAs in the Escherichia coli Ribosome
Authors:
- 16 February 1996
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 271 (5251) , 1000-1002
Abstract
Transfer RNA (tRNA) molecules play a crucial role in protein biosynthesis in all organisms. Their interactions with ribosomes mediate the translation of genetic messages into polypeptides. Three tRNAs bound to the Escherichia coli 70 S ribosome were visualized directly with cryoelectron microscopy and three-dimensional reconstruction. The detailed arrangement of A- and P-site tRNAs inferred from this study allows localization of the sites for anticodon interaction and peptide bond formation on the ribosome.Keywords
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