Equipment Setup for Fluorescence Imaging with One-Nanometer Accuracy (FIONA)
Authors:
- Paul R. SelvinPSPaul R. Selvin
- Tyler LougheedTLTyler Lougheed
- Melinda Tonks HoffmanMHMelinda Tonks Hoffman
- Hyokeun ParkHPHyokeun Park
- Hamza BalciHBHamza Balci
- Benjamin H. BlehmBBBenjamin H. Blehm
- Erdal ToprakETErdal Toprak
- 1 October 2007
- journal article
- Published by Cold Spring Harbor Laboratory in Cold Spring Harbor Protocols
- Vol. 2007 (10) , pdb.ip45
Abstract
INTRODUCTION: FIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. This article describes procedures for setting up the equipment necessary for FIONA and achieving total internal reflection (TIR).Keywords
This publication has 1 reference indexed in Scilit:
- Polarization effect on position accuracy of fluorophore localizationOptics Express, 2006