Phospholipid composition of canola oils during the early stages of processing as measured by TLC with flame lonization detector

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Abstract
Canola oils at initial stages of processing from different crushing plants were analyzed for phosphatides. The major phospholipid components identified and quantified in refined canola oils were phosphatidic acid and phos‐phatidylinositol. Phosphatidic acid was the main phosphorus component identified in solvent extracted canola oil samples. The two‐dimensional separation that was used combined classical thin‐layer chromatography with quantitation on chromarods in an Iatroscan with a flame ionization detector. Phosphatides quantitated with this procedure ranged from 0.1 to 20 μg with a coefficient of variation of 4.4 to 7.2%. Using the modified procedure, recoveries of better than 90% were obtained for all phospholipids analyzed.

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