Plasma membrane Fe2‐transferrin reductase and iron uptake in K562 cells are not directly related
Authors:
Open Access
- 1 September 1990
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 192 (2) , 475-480
Abstract
Receptor‐mediated endocytosis and recycling of transferrin is partly inhibited by the ferrous iron chelator bipyridine, which almost completely blocks iron uptake. Bipyridine causes iron release at the cell surface, but inhibition of iron uptake is also due to a blockage of its passage through the endosomal membrane. The rate of release of iron to bipyridine is decreased by the competing electron acceptor ferricyanide and by amiloride, but not by iron uptake inhibiting acidotropic amines.Transferrin reduction at the plasma membrane may be artificially induced by presence of a ferrous chelator and caused by low‐affinity transmembrane NAD(P)H oxidoreductase.Keywords
This publication has 44 references indexed in Scilit:
- Diferric transferrin reductase in Plasmodium falciparum-infected erythrocytesBiochemical and Biophysical Research Communications, 1989
- Diferric transferrin reduction stimulates the Na+H+ antiport of HeLa cellsBiochemical and Biophysical Research Communications, 1987
- Transplasmalemma electron transport from cells is part of a diferric transferrin reductase systemBiochemical and Biophysical Research Communications, 1986
- Transplasma-membrane redox systems in growth and developmentBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1985
- Intravesicular pH and iron uptake by immature erythroid cellsJournal of Cellular Physiology, 1984
- Separation of Fe+3 from transferrin in endocytosisFEBS Letters, 1983
- Chelator-mediated iron efflux from reticulocytesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1983
- Co-migration and internalization of transferrin and its receptor on K562 cells.Journal of Cell Biology, 1983
- Amiloride blocks cell-free protein synthesis at levels attained inside cultured rat hepatocytesBiochemical and Biophysical Research Communications, 1982
- Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycolurilBiochemical and Biophysical Research Communications, 1978