Liquid nitrogen storage of yeast cultures I. Survival, and literature review of the preservation of fungi at ultralow temperatures

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Abstract
All 20 yeast strains of 17 species tested survived 75 days (the length of the experimental period) in liquid nitrogen at-196 C. The components of the more protective of the two freezing media used were (w/v) malt extract 2.5 %, yeast extract 0.25 %, peptone 0.5 %, calf serum 15 % (v/v) and dimethyl sulfoxide 10 % (v/v). Viability of the cells in this medium after rapid uncontrolled freezing and thawing in sealed plastic ampoules ranged from 2 % to 98 % (average 67 %) compared with the viability of the cultures before freezing. In only 4 strains was survival lower than 50 %. (90 references).

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