Dengue virus (DENV) infection is caused by Aedes sp. mosquitoes such as A. aegypti, A. albopictus, and A. scutellaris. DENV has been reported to possess a cell tropism consisting of mast cells, fibroblasts, dendritic cells, macrophages, langerhans, and keratinocytes. Viral replication consists of various stages such as attachment, fusion, replication of genetic material, assembly, and virus release. Fusion inhibitors often act to interfere with the endocytosis process mediated by parts on the virus and host receptor. However, few previous studies have used in silico approaches to screen candidate DENV fusion inhibitors with bioactive compounds. Several volatile compounds such as flavonoids contained in Sonchus arvensis L. leaf extracts are predicted to have potential for treatment. Therefore, this study aims to determine the activity of volatile compounds from Sonchus arvensis L. to inhibit DENV fusion through in silico approach. The in silico methods used were ligand collection, protein retrieval, druglikeness prediction, virtual screening, molecular interaction analysis, molecular dynamics, toxicity profiling, and antiviral probability. The results showed that volatile compounds from Sonchus arvensis L. consisting of Hexadecanoic acid, ethyl ester, Phytol, 1,2-benzenedicarboxylic acid, bis(2-methylpropyl)ester, and Benzoic acid, 2-ethylhexyl ester could inhibit DENV fusion through binding to DENV proteins.