Exploring the Protective Effects of Phaeodactylum tricornutum Extract on LPS-Treated Fibroblasts
Open Access
Abstract
Background: Microalgal extracts are an important source of bioactive compounds with antioxidant and anti-inflammatory properties that can be used in cosmetics. The microalgae Phaeodactylum tricornutum (PT) is known for its high content of omega-3 fatty acids, which are known to attenuate inflammation. Here, we explore the effects of aqueous microencapsulated extract of PT on lipopolysaccharide (LPS)-stimulated normal human dermal fibroblasts (NHDF) to underline its application as an active ingredient in cosmetics. Methods: We assessed cell viability using MTT assay, so as to target any potential cytotoxicity of the extract. Moreover, with the aid of RT-qPCR, we studied the transcript accumulation of genes involved in cell antioxidant response, cell proliferation, and inflammation. Results: Our results revealed that the hydrolyzed rice flour-encapsulated (HRF) PT extract promotes anti-inflammatory and antioxidant response, increasing cell proliferation in NHDF cells. Conclusions: Our data indicate a promising use of HRF-encapsulated PT extract in cosmetics by reducing skin inflammation.Keywords
This publication has 39 references indexed in Scilit:
- Gelam Honey Protects against Gamma-Irradiation Damage to Antioxidant Enzymes in Human Diploid FibroblastsMolecules, 2013
- BindaritCell Cycle, 2012
- Why does chronic inflammation persist: An unexpected role for fibroblastsImmunology Letters, 2011
- Cellular response to infrared radiation involves retrograde mitochondrial signalingFree Radical Biology and Medicine, 2007
- Micro-algae as a source of proteinBiotechnology Advances, 2007
- Age‐dependent response of primary human dermal fibroblasts to oxidative stress: cell survival, pro‐survival kinases, and entrance into cellular senescenceWound Repair and Regeneration, 2005
- Kiwi fruit (Actinidia chinensis L.) polysaccharides exert stimulating effects on cell proliferation via enhanced growth factor receptors, energy production, and collagen synthesis of human keratinocytes, fibroblasts, and skin equivalentsJournal of Cellular Physiology, 2004
- Gingival and dermal fibroblasts produce interleukin‐1β converting enzyme and interleukin‐1β but not interleukin‐18 even after stimulation with lipopolysaccharideJournal of Cellular Physiology, 2003
- Anti‐inflammatory and immunomodulatory activities of polysaccharide from Chlorella stigmatophora and Phaeodactylum tricornutumPhytotherapy Research, 2003
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001