Penicillin Acylase Catalysed Synthesis of Ampicillin in Hydrophilic Organic Solvents
Authors:
- 3 June 2003
- journal article
- research article
- Published by Wiley in Advanced Synthesis & Catalysis
- Vol. 345 (6-7) , 797-801
Abstract
Penicillin acylase (EC 3.5.1.11) from Alcaligenes faecalis, immobilised as a cross‐linked enzyme aggregate (CLEA), catalysed the synthesis of ampicillin in water‐miscible organic solvents at low water concentrations. Below 4% water (v/v) no reaction was observed, showing the crucial role of water in maintaining the activity of penicillin acylase. The initial value of S/H was strongly affected by the nature of the solvent, but the effect of the water content was slight in the studied range of 4 to 15%. A reaction in acetonitrile containing 8% water afforded ampicillin in up to 86% yield.Keywords
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