Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH)
Authors:
- 26 October 2021
- book chapter
- Published by Springer Nature
- Vol. 2404, 207-218
Abstract
MicroRNA capture affinity technology (miR-CATCH) uses affinity capture biotinylated antisense oligonucleotides to co-purify a target transcript together with all its endogenously bound miRNAs. The miR-CATCH assay is performed to investigate miRNAs bound to a specific mRNA. This method allows to have a total vision of miRNAs bound not only to the 3′UTR but also to the 5′UTR and Coding Region of target messenger RNAs (mRNAs).Keywords
This publication has 17 references indexed in Scilit:
- Reduced miR-659-3p Levels Correlate with Progranulin Increase in Hypoxic Conditions: Implications for Frontotemporal DementiaFrontiers in Molecular Neuroscience, 2016
- miR-CATCH: MicroRNA Capture Affinity Technology2014
- Isolation and identification of cell-specific microRNAs targeting a messenger RNA using a biotinylated anti-sense oligonucleotide capture affinity techniqueNucleic Acids Research, 2013
- Evaluating the MicroRNA Targeting Sites by Luciferase Reporter Gene Assay2012
- Unipro UGENE: a unified bioinformatics toolkitBioinformatics, 2012
- Silencing of microRNA families by seed-targeting tiny LNAsNature Genetics, 2011
- A new vector, based on the PolII promoter for the U1 snRNA gene, for the expression of siRNAs in mammalian cellsMolecular Therapy, 2004
- Mfold web server for nucleic acid folding and hybridization predictionNucleic Acids Research, 2003
- Thermodynamics−Structure Relationship of Single Mismatches in RNA/DNA DuplexesBiochemistry, 2000
- Basic local alignment search toolJournal of Molecular Biology, 1990