Defining the mutation signatures of DNA polymerase θ in cancer genomes

Authors:
Open Access
Publisher website
Google Scholar
Abstract
DNA polymerase theta (POLQ)-mediated end joining (TMEJ) is a distinct pathway for mediating DNA double-strand break (DSB) repair. TMEJ is required for the viability of BRCA-mutated cancer cells. It is crucial to identify tumors that rely on POLQ activity for DSB repair, because such tumors are defective in other DSB repair pathways and have predicted sensitivity to POLQ inhibition and to cancer therapies that produce DSBs. We define here the POLQ-associated mutation signatures in human cancers, characterized by short insertions and deletions in a specific range of microhomologies. By analyzing 82 COSMIC (Catalogue of Somatic Mutations in Cancer) signatures, we found that BRCA-mutated cancers with a higher level of POLQ expression have a greatly enhanced representation of the small insertion and deletion signature 6, as well as single base substitution signature 3. Using human cancer cells with disruptions of POLQ, we further show that TMEJ dominates end joining of two separated DSBs (distal EJ). Templated insertions with microhomology are enriched in POLQ-dependent distal EJ. The use of this signature analysis will aid in identifying tumors relying on POLQ activity.
Funding Information
  • Institute for Basic Science (IBS-R022-A1)
  • National Cancer Institute (P01 CA193124, R03 CA219434, P30-CA016672)
  • MD Anderson Research Trust
  • Grady F. Saunders Ph.D. Distinguished Research Professorship
  • Cancer Prevention and Research Institute of Texas (RP150102, RP170002)

This publication has 51 references indexed in Scilit: