Thermoelectric‐based temperature‐controlling system for in‐tube solid‐phase microextraction

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Abstract
A temperature‐controlling device for in‐tube solid‐phase microextraction was developed based on thermoelectric cooling and heating. This device can control the temperature of the capillary column from 0 to 100°C by applying a voltage to a Peltier cooler or stainless steel tube. The extraction temperatures for angiotensin I, propranolol, and ranitidine were optimized. In all cases, setting the temperature to 10°C for extraction achieved the best extraction efficiency. Desorption showed minimum peak broadening at 70°C, contributing to better chromatographic performance. Propranolol was selected as a model compound to compare the performance of temperature‐controlled in‐tube solid‐phase microextraction at optimized conditions. Calibration curves exhibited good linearity (R2 > 0.999) over the studied range, and the limit of detection and limit of quantification were about three times lower than those obtained at standard conditions (30°C extraction and desorption).
Funding Information
  • Natural Sciences and Engineering Research Council of Canada
  • Ontario Ministry of Research and Innovation, and Scholarship funds (Y.Y.)
  • the China Scholarship Council

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