Extremophilic and common fungi in acid brines and their halite

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Abstract
Studies of microorganisms in extreme Mars-analog environments have generally overlooked fungi. Here, we document fungi in lake waters, slime, and halite of the acid-saline Lakes Magic and Gneiss in Western Australia with pH 1.4–3.5 and 7–32% total dissolved solids (TDS). Both extremotolerant fungi, including ascomycete Parengyodontium torokii, and relatively common fungi (mesophilic), including Penicillium breviocompactum and Trametes pubescens, were present. Our discovery of P. torokii in halite is among the first known fungal examples of such preservation, and we propose that it has the biological traits of a generalist species. Nine strains of the dominant P. torokii fungi were tested for growth on diverse salts. The presence of mesophilic fungal saprotrophs in these lakes, along with extremophilic fungi, algae, bacteria, and archaea, suggests transport of the former into indigenous lake populations. This reveals a distinction between habitability and preservation potential; not all biosignatures in lake waters or their halite represent organisms that were active in situ. Our results suggest that searches for biosignatures in extreme waters and salt minerals on Earth and Mars should include the possibility of fungi. Additionally, interpretations of microbial communities in both modern brines and the rock record should consider the likelihood of mixed indigenous and transported taxa.
Funding Information
  • National Aeronautics and Space Administration (80NSSC18K1286, 80NSSC20K0235)
  • National Science Foundation (EAR-0902250, EAR-1317138)
  • The Slovenian Research and Innovation Agency (ARIS P1-0198, MRIC UL, 10-0022, P4-0432)