Kathleen Counter Benison

Studies of microorganisms in extreme Mars-analog environments have generally overlooked fungi. Here, we document fungi in lake waters, slime, and halite of the acid-saline Lakes Magic and Gneiss in Western Australia with pH 1.4–3.5 and 7–32% total dissolved solids (TDS). Both extremotolerant fungi, including ascomycete Parengyodontium torokii, and relatively common fungi (mesophilic), including Penicillium breviocompactum and Trametes pubescens, were present. Our discovery of P. torokii in halite is among the first known fungal examples of such preservation, and we propose that it has the biological traits of a generalist species. Nine strains of the dominant P. torokii fungi were tested for growth on diverse salts. The presence of mesophilic fungal saprotrophs in these lakes, along with extremophilic fungi, algae, bacteria, and archaea, suggests transport of the former into indigenous lake populations. This reveals a distinction between habitability and preservation potential; not all biosignatures in lake waters or their halite represent organisms that were active in situ. Our results suggest that searches for biosignatures in extreme waters and salt minerals on Earth and Mars should include the possibility of fungi. Additionally, interpretations of microbial communities in both modern brines and the rock record should consider the likelihood of mixed indigenous and transported taxa.

Water activity is an important characteristic for describing unusual waters and is a determinant of habitability for microorganisms. However, few empirical studies of water activity have been done for natural waters exhibiting an extreme chemistry. Here, we investigate water activity for acid brines from Western Australia and Chile with pH as low as 1.4, salinities as high as 32% total dissolved solids, and complex chemical compositions. These acid brines host diverse communities of extremophilic microorganisms, including archaea, bacteria, algae, and fungi, according to metagenomic analyses. For the most extreme brine, its water activity (0.714) was considerably lower than that of saturated (pure) NaCl brine. This study provides a thermodynamic insight into life within end-member natural waters that lie at, or possibly beyond, the very edge of habitable space on Earth.

The climatic, biotic, and tectonic events of the Permian are amongst the most profound in Earth history. Global orogeny leading to Pangaean assembly culminated by middle Permian time, and included multiple orogenic belts in the equatorial Central Pangaean Mountains, from the Variscan-Hercynian system (east) to the Ancestral Rocky Mountains (west). Earth’s penultimate global icehouse peaked in early Permian time, transitioning to full greenhouse conditions by late Permian time, thus archiving the only example of icehouse collapse on a fully vegetated Earth. The Late Paleozoic Icehouse was the longest and most intense glaciation of the Phanerozoic, with hypothesized low-elevation glaciation posited for both eastern and western tropical Pangaea during early Permian time. Reconstructions of atmospheric composition record the lowest CO2 and highest O2 levels of the Phanerozoic, with average CO2 levels comparable to the Quaternary, rapidly warming climate. Fundamental shifts occurred in atmospheric circulation: a global megamonsoon developed and the tropics became anomalously arid with time. Extreme environments are well documented in the form of voluminous dust deposits, acid-saline lakes and groundwaters, extreme continental temperatures and aridity, and major extinctions/extirpations, ultimately culminating at the Permo-Triassic boundary with the largest extinction of Earth history.We seek to elucidate paleoclimatic conditions and forcings through the Permian at temporal scales ranging from the millennial to the Milankovitch  and beyond by acquiring continuous core in continental lowlands known to harbor stratigraphically complete records dominated by loess and lacustrine strata. We have identified sites in the western U.S. and Europe as the key sites globally to achieve our objectives, as these represent the western and eastern limits, respectively of the Pangaean tropics. Identified sites harbor arguably the most complete continental Permian sections in the paleoequatorial region, with adjacent paleo-uplands hypothesized to have hosted glaciation. We will also address the nature and character of the modern and fossil microbial biosphere, Mars-analog conditions, and exhumation histories of source regions.

Abundant resources and efforts have been employed in the search for life on Mars. Satellites, landers, and rovers have tested atmospheric gases, general sediment and rock compositions, and images of Mars surface in an effort to detect biosignatures left by any possible modern or ancient life. Chloride and sulfate minerals suggestive of past acid saline lakes have been found on Mars. In terrestrial acid brine environments, these minerals trap microorganisms and organic compounds and preserve them within fluid inclusions and as solid inclusions for long geologic time periods. Some cells remain viable, especially in the isolated, microscopic aqueous environments of fluid inclusions. Fluid inclusions in these same saline minerals on Mars have yet to be examined. This paper describes petrographic and geochemical methods that have been used recently to detect and make general identifications of microorganisms and organic compounds preserved in modern and Permian Mars-analog acid saline lake halite and gypsum. It then makes recommendations for how Martian chemical sediments could be examined for these biosignatures, both by rovers and in returned samples. This new protocol for the examination of Martian chemical sediments and sedimentary rocks may provide the next step for detection of any preserved biosignatures on Mars.

Lake Magic is one of the most extreme of hundreds of ephemeral acid-saline lakes in southern Western Australia. It has pH as low as 1.7, salinity as high as 32% total dissolved solids, temperatures ranging from 0°C to 50°C, and an unusually complex aqueous composition. Optical petrography, UV-vis petrography, and laser Raman spectrometry were used to detect microorganisms and organic compounds within primary fluid inclusions in modern bedded halite from Lake Magic. Rare prokaryotes appear as 1–3 μm, bright cocci that fluoresce green with UV-vis illumination. Dimpled, 5–7 μm yellow spherules that fluoresce blue with UV-vis illumination are interpreted as Dunaliella algae. Yellow-orange beta-carotene crystals, globules, and coatings are characterized by orange-red fluorescence and three distinct Raman peaks. Because acid saline lakes are good Mars analogues, the documentation of prokaryotes, eukaryotes, and organic compounds preserved in the halite here has implications for the search for life on Mars. Missions to Mars should incorporate such in situ optical and chemical examination of martian evaporites for possible microorganisms and/or organic compounds in fluid inclusions. Key Words: Acid—Extremophiles—Western Australia—Fluid inclusions—Lake Magic—Dunaliella. Astrobiology 13, 850–860.