Mining and Genetic Mapping of a Novel Powdery Mildew Resistance Gene, PmKu-2013, Identified in Aegilops tauschii
Open Access
Abstract
Wheat powdery mildew is a fungal disorder caused by Blumeria graminis f. sp. tritici (Bgt) and is a severe and significant threat to the yield and quality of its host. The most practical and environmentally friendly approach to controlling this disease is through resistance gene identification to develop resistant varieties. Wild germplasm relatives of wheat are a valuable reservoir of genes contributing to resistance against powdery mildew. In our study, we identified the Aegilops tauschii germplasm “KU-2013”, exhibiting seedling resistance to Bgt isolate E09 following hexaploidization. Genetic analysis and chromosomal localization of the powdery mildew resistance gene in doubled haploid (DH) KU-2013 indicated that the disease resistance gene in DHKU-2013 is governed by a dominant gene situated in 5DS, tentatively named PmKu-2013. Following the analysis of PmKu-2013 relative to the genes at the Pm2 locus, it was inferred that PmKu-2013 represented a distinct novel gene separate from Pm2. Using molecular marker analysis, PmKu-2013 was found to be ultimately mapped between the sdau5DS5-3 and sdau5DS6-1 markers, with genetic distances of 0.6 cM and 1.3 cM, respectively. Using markers tightly linked to PmKu-2013, the genotypes of core wheat varieties from various regions were identified, laying the foundation for the transfer and utilization of PmKu-2013 in molecular-assisted selection (MAS) for breeding.Keywords
Funding Information
- National Key Research and Development Program of China (2021YFD1200602-5, 31801352, 2023LZGC022)
- National Natural Science Foundation of China (2021YFD1200602-5, 31801352, 2023LZGC022)
- Agricultural Variety Improvement Project of Shandong Province (2021YFD1200602-5, 31801352, 2023LZGC022)
This publication has 49 references indexed in Scilit:
- Genetic mapping of the wheat leaf rust resistance gene Lr19 and development of translocation lines to break its linkage with yellow pigmentTheoretical and Applied Genetics, 2023
- Genetic basis of resistance against powdery mildew in the wheat cultivar “Tabasco”Molecular Breeding, 2023
- Development of breeder chip for gene detection and molecular-assisted selection by target sequencing in wheatMolecular Breeding, 2023
- Molecular tagging of a new broad-spectrum powdery mildew resistance allele Pm2c in Chinese wheat landrace NiaomaiTheoretical and Applied Genetics, 2015
- Functional markers in wheat: current status and future prospectsTheoretical and Applied Genetics, 2012
- Rye-derived powdery mildew resistance gene Pm8 in wheat is suppressed by the Pm3 locusTheoretical and Applied Genetics, 2011
- Molecular identification of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var. dicoccoides)Theoretical and Applied Genetics, 2009
- Development of wheat near-isogenic lines for powdery mildew resistanceTheoretical and Applied Genetics, 2005
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Genetic and population study of a Y-linked tetranucleotide repeat DNA polymorphism with a simple non-isotopic techniqueHuman Genetics, 1993