DNA G-Quadruplex Is a Transcriptional Control Device That Regulates Memory
Authors:
- Paul R. MarshallPMPaul R. Marshall
- Qiongyi ZhaoQZQiongyi Zhao
- Wei-Siang LiauWLWei-Siang Liau
- Yujin LeeYLYujin Lee
- Dean BasicDBDean Basic
- Esmi L. ZajaczkowskiEZEsmi L. Zajaczkowski
- Laura J. LeightonLLLaura J. Leighton
- Sachithrani U. MadugalleSMSachithrani U. Madugalle
- Mason MusgroveMMMason Musgrove
- Marcin KielarMKMarcin Kielar
Open Access
- 28 February 2024
- journal article
- research article
- Published by Society for Neuroscience in The Journal of Neuroscience
- Vol. 44 (15)
Abstract
The conformational state of DNA fine-tunes the transcriptional rate and abundance of RNA. Here, we report that G-quadruplex DNA (G4-DNA) accumulates in neurons, in an experience-dependent manner, and that this is required for the transient silencing and activation of genes that are critically involved in learning and memory in male C57/BL6 mice. In addition, site-specific resolution of G4-DNA by dCas9-mediated deposition of the helicase DHX36 impairs fear extinction memory. Dynamic DNA structure states therefore represent a key molecular mechanism underlying memory consolidation. One-Sentence Summary: G4-DNA is a molecular switch that enables the temporal regulation of the gene expression underlying the formation of fear extinction memory.Keywords
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Funding Information
- ARC (GA142202)
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