At-ore1 Gene Induces Distinct Novel H2O2-NACs Signaling in Regulating the Leaf Senescence in Soybeans (Glycine max L.)
Open Access
Abstract
Senescence is modulated by ORESARA1 (ORE1), a NAC transcription factor that interacts with hormones to fully induce senescence. The At-ore1 gene acts as a suppressor of leaf senescence; however, its exact role in this respect has not been clearly defined. In this study, the function of At-ore1 during leaf senescence was analyzed in soybeans. The precocious leaf senescence of the ore1-1 line was associated with greater chlorophyll loss, leaf necrosis, and redox imbalance in the early vegetative stage during the hyper-accumulation of endogenous abscisic acid (ABA) by enhancing the expression of GmNECD3-related ABA synthesis. At-ore1 induced ABA regulation of the H2O2-GmARF2-GmNAC081 signaling circuit, which relays the At-ore1-induced cell death signal mediation to the caspase-1-like vacuolar processing enzyme (VPE) expression, triggering programmed cell death. In contrast, it was found that At-ore1 functions in IAA to delay leaf-senescence-mediated suppression of the expression of ABA, ROS, and senescence-associated gene 39 (GmSAG39). The IAA-induced GmNAC065 expression controls soybean leaves’ longevity, as discovered by screening At-ore1 expression in ore1-6 for a more stay-green leaf phenotype by helping to increase seed yields. These results uncover a mechanism that modulates ore1 plants’ amplitude expression involved in the ABA/IAA balance in the activation of GmNAC081- or GmNAC065-dependent H2O2 levels, which are crucial in the senescence or delayed leaf senescence of soybeans.Keywords
Funding Information
- Ministry of Science and Technology (MOST) of Vietnam (NĐT.49.KR.18)
This publication has 70 references indexed in Scilit:
- ATAF1 transcription factor directly regulates abscisic acid biosynthetic gene NCED3 in Arabidopsis thalianaFEBS Open Bio, 2013
- Differential Gene Expression in Soybean Leaf Tissues at Late Developmental Stages under Drought Stress Revealed by Genome-Wide Transcriptome AnalysisPLOS ONE, 2012
- The NAC domain-containing protein, GmNAC6, is a downstream component of the ER stress- and osmotic stress-induced NRP-mediated cell-death signaling pathwayBMC Plant Biology, 2011
- Auxin Response Factor2 (ARF2) and Its Regulated Homeodomain Gene HB33 Mediate Abscisic Acid Response in ArabidopsisPLOS Genetics, 2011
- YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thalianaJournal of Experimental Botany, 2011
- High-Resolution Temporal Profiling of Transcripts duringArabidopsisLeaf Senescence Reveals a Distinct Chronology of Processes and RegulationThe Plant Cell, 2011
- ORS1, an H2O2-Responsive NAC Transcription Factor, Controls Senescence in Arabidopsis thalianaMolecular Plant, 2011
- Auxin response factor 2 (ARF2) plays a major role in regulating auxin-mediated leaf longevityJournal of Experimental Botany, 2010
- A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescenceThe Plant Journal, 2010
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001