Angiotensin II induces cell growth and IL-6 mRNA expression through the JAK2-STAT3 pathway in rat cerebellar astrocytes
Open Access
- 1 April 2012
- journal article
- Published by Taylor & Francis in JAK-STAT
- Vol. 1 (2) , 83-89
Abstract
The pleiotrophic effects of angiotensin II (Ang II) play important roles in astrocyte growth and inflammatory responses. We investigated whether Ang II induces astrocyte growth and interleukin-6 (IL-6) mRNA expression in rat cerebellar astrocytes through Janus kinase 2-signal transduction activator of transcription (JAK2-STAT3). Ang II increased JAK2 and STAT3 phosphorylation in a time- and a dose-dependent manner. One hundred nanomolar Ang II induced maximal phosphorylation of both JAK2 and STAT3 between 15 min and 30 min. The Ang II-mediated phosphorylation of both JAK2 and STAT3 was blocked by AG490, a selective JAK2 inhibitor. Losartan, a selective AT1 receptor antagonist, inhibited Ang II-mediated JAK2 and STAT3 phosphorylation, while pretreatment with an AT2 receptor blocker, PD123319, was ineffective. Ang II increased the mRNA expression of IL-6 in a concentration-and time-dependent manner. Maximal IL-6 mRNA expression occurred with 100 nM Ang II, and the peak effect occurred in a biphasic manner a...Keywords
This publication has 46 references indexed in Scilit:
- Anti-Inflammatory Effects of Angiotensin Receptor Blockers in the Brain and the PeripheryCellular and Molecular Neurobiology, 2009
- Angiotensin II cell signaling: physiological and pathological effects in the cardiovascular systemAmerican Journal of Physiology-Cell Physiology, 2007
- Angiotensin II regulates the synthesis of proinflammatory cytokines and chemokines in the kidneyKidney International, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Angiotensin Induces Inflammatory Activation of Human Vascular Smooth Muscle CellsArteriosclerosis, Thrombosis, and Vascular Biology, 1999
- Angiotensin II activates distinct signal transduction pathways in astrocytes isolated from neonatal rat brainGlia, 1997
- Angiotensin-(1-7) Inhibits Vascular Smooth Muscle Cell GrowthHypertension, 1996
- The role of tyrosine phosphorylation in angiotensin II-mediated intracellular signallingCardiovascular Research, 1995
- Direct stimulation of Jak/STAT pathway by the angiotensin II AT1 receptorNature, 1995
- Astrocytes Synthesize Angiotensinogen in BrainScience, 1988