Yongchang Liu

Background: The functional role of GhZFP8 (a zinc finger protein gene) in plant growth and development remains unclear. Methods: This study investigated phenotypic and transcriptomic changes in GhZFP8-transgenic vs. wild-type Arabidopsis to clarify GhZFP8’s biological functions. Phenotypes of two transgenic lines (ZFP8_7, ZFP8_21) were observed. Transcriptome sequencing (ZFP8_7 vs. wild-type, 3 biological replicates/sample) was performed, with quality assessment (coverage, depth, alignment rate). Differential genes were screened by |log2(fold change)| > 1 and FDR < 0.05; GO/KEGG enrichment analyses were conducted. Results: Transgenic lines showed slower growth, higher trichome density, ectopic silique trichomes, and reduced fertility. Sequencing quality was satisfactory (97.42% exonic alignment). Most differential genes were highly expressed in wild-type plants, with more downregulated than upregulated genes. Upregulated genes enriched in stimulus response regulation, plant cell walls, and methyltransferase activity (GO); estrogen signaling/tyrosine metabolism (KEGG). Downregulated genes enriched in cell wall/phenylpropanoid biosynthesis (GO); glycosyltransferase/plant MAPK pathways (KEGG). Conclusions:GhZFP8 overexpression induces significant phenotypic changes and alters gene expression in Arabidopsis. Findings provide insights into GhZFP8’s effects, supporting further study of its functional mechanisms.

Cotton is one of the most important natural fibers used in the textile industry worldwide. It is important to identify the key factors involved in cotton fiber development. In this study, zinc finger protein8 (GhZFP8) encoding a C₂H₂ transcription factor (TF) was cloned from cotton. qPCR showed that the transcripts of GhZFP8 in cotton were detected in the leaves and fibers at 3, 6, and 30 days post-anthesis (DPA), but not in the roots, stems, or flowers. The overexpression of GhZFP8 increased the trichome number on the siliques, leaves, and inflorescence, but inhibited the growth. The expression of trichome development and cell-elongation-related genes decreased obviously in GhZFP8 overexpressor Arabidopsis. Indole-3-acetic acid (IAA) and 1-Aminocyclopropanecarboxylic acid (ACC) contents were much higher in GhZFP8 overexpressors than that found in the wild type, but the gibberellin (GA) content was lower. The interference of GhZFP8 in cotton caused smaller bolls and shorter fibers than that of the control. The results of DNA affinity purification (DAP)-seq showed that GhZFP8 could bind to the promoter, exon, intron, and intergenic region of the target genes, which are involved in photosynthesis, signal transduction, synthesis of biomass, etc. Our findings implied that GhZFP8 processed multiple biological functions and regulated the development of cotton fiber.

Norcantharidin (NCTD), a demethylated derivative of cantharidin (defensive toxin of blister beetles), has been reported to exhibit insecticidal activity against various types of agricultural pests. However, NCTD applications are limited by its poor water solubility and high dosage requirement. Nanoemulsions have attracted much attentions due to the transparent or translucence appearance, physical stability, high bioavailability and non-irritant in nature. In general, nanoemulsions with small droplet size can enhance the bioavailability of drugs, whereas this phenomenon is likely system dependent. In present study, NCTD nanoemulsions were developed and optimized to evaluate and improve the insecticidal activity of NCTD against Plutella xylostella (Lepidotera: Plutellidae) by a spontaneous emulsification method. Triacetin, Cremophor EL and butanol were selected as the constituents of NCTD nanoemulsions via solubility determination, emulsification efficiency and ternary phase diagram construction. Insecticidal activity of NCTD nanoemulsion was associated with the content of surfactant and cosurfactant: (1) Higher effective toxicity exhibited at Smix (surfactant to cosurfactant mass ratio) = 3:1 that may be associated with the changes in interfacial tension; (2) NCTD nanoemulsion at 3:7 < SOR (surfactant to oil mass ratio) < 6:4 was more effective at lower surfactant level, which was attributed to the relatively slow diffusion rate of NCTD hindering by excess surfactant. Interestingly, nanoemulsions with smaller droplets were not found to be more effective in our study. The optimized NCTD nanoemulsion (triacetin/Cremophor EL/butanol (60/20/20, w/w)) exhibited effective insecticidal activity (LC50 60.414 mg/l, LC90 185.530 mg/l, 48 h) than the NCTD acetone solution (LC50 175.602 mg/L, LC90 303.050 mg/L, 48 h). Spontaneous emulsifying nanoemulsion employed to formulate this poor water-soluble pesticide is a potential system for agriculture application.