Muhammad Agus Suprayudi

Thalassiosira sp. is a significant marine diatom utilized as live feed in aquaculture; however, its culture productivity frequently remains below optimal levels. The present study evaluated the growth performance and culture quality of Thalassiosira sp. under two parallel cultivation strategies: a mixotrophic system supplemented with sucrose and a system supplemented with Indole-3-Acetic Acid (IAA). Both cultivation schemes were maintained for seven days under identical environmental conditions. Sucrose was administered at concentrations ranging from 0.25 to 1 g/L, while IAA was applied at 2.5 to 10 mg/L. Cell density, specific growth rate, dry biomass, and total Vibrio count were measured. Sucrose supplementation, particularly at 1 g/L, resulted in increased growth and biomass accumulation but was accompanied by elevated Vibrio abundance. IAA supplementation also promoted growth and biomass production, with the most pronounced effect observed at 10 mg/L, although it did not reduce bacterial proliferation. These findings indicate that both sucrose and IAA can enhance Thalassiosira sp. culture productivity under controlled conditions, but effective microbial management remains essential for practical implementation.

Efficient dietary protein utilization is essential to reduce feed costs and environmental impacts in sustainable aquaculture. This study aimed to evaluate the effects of cinnamaldehyde (CA) supplementation in feed with various energy-to-protein (E:P) ratios on the chemical composition of Pangasianodon hypophthalmus. The five feed formulas that made up the treatment feed were as follows: 28:13-C0 (28 % Protein with an E:P ratio of 13 and CIN 0 g kg-1); 25:14-C1 (25 % protein with an E:P ratio of 14 and CIN 1.2 g kg-1); 25:14-C2 (25 % protein with an E:P ratio of 14 and CIN 1.7 g kg-1); 25:15-C1 (25 % protein with an E:P ratio of 15 and CIN 1.2 g kg-1); and 25:15-C2 (25 % Protein with an E:P ratio of 15 and CIN 1.7 g kg-1). Striped catfish weighing 28.06 ± 0.19 g were placed in a hapa (2 × 1 × 1 m3) at a density of 25 fish per cage. Fish were fed to apparent satiation three times daily for 60 d. The 25:14-C2, 25:15-C1, and 25:15-C2 diets increased albumin levels and reduced cholesterol, while 25:15-C2 also yielded the highest total protein and lowest triglyceride levels. Growth performance and feed efficiency were comparable among 28:13-C0, 25:14-C2, 25:15-C1, and 25:15-C2 (final weight: 141.62–143.75 g; FCR: 1.16–1.19). Protein efficiency ratio was highest in 25:15-C1 and 25:15-C2, whereas protein retention peaked in 25:14-C2. The hepatosomatic index was elevated in 25:14-C1, 25:14-C2, and 25:15-C1. Body lipid content was highest in 25:15-C1, while muscle lipid content was lowest in 25:14-C2 and 25:15-C1. Reducing dietary protein from 28 % to 25 % did not compromise growth performance at the E:P ratio level of 15 with a supplementation of 1.2 g kg-1 CIN.

Copper (Cu-O) is a micromineral required in small amounts in the body, yet it plays an important role in the metabolism and health of fish. This study aimed to evaluate the growth performance of tilapia (Oreochromis sp.) fed diets supplemented with organic copper (Cu-O). Four treatments were tested: K (control, without Cu-O), Cu-O 1 (1 mg kg⁻¹ Cu-O), Cu-O 2 (2 mg kg⁻¹ Cu-O), and Cu-O 3 (3 mg kg⁻¹ Cu-O). Fifteen tilapia with an average initial weight of 8.75 ± 0.02 g were reared in aquaria (60 × 50 × 40 cm, water depth 30 cm) for 60 days. Fish were fed to satiation three times daily. The results showed that the growth parameters of tilapia fed Cu-O-supplemented diets were significantly higher (P < 0.05) than those of the control. The optimal dose of Cu-O supplementation was found to be 2 mg kg⁻¹ feed; subsequent studies should investigate interspecies variations and refine dosage recommendations accordingly.

Raw material cost including protein sources account for at least 70% of total aquafeed production cost. Protein is among the top three most expensive nutrients to supply in aquaculture diets, after energy and phosphorus. It is imperative to identify effective and practical strategies to ensure that African catfish (Clarias gariepinus; Burchell, 1822) utilizes amino acid intake for optimum growth. The ever-increasing cost-price squeeze between aquaculture input costs and output prices have forced aquafeed to reduce dietary nutrient specifications particularly amino acids. This occurs against a backdrop of sustainability concerns regarding fish meal as a protein source. This work studied 450 C. gariepinus in 15 experimental aquariums for a 62-day culture period. Implementing an experimental design with five fish meal-free dietary treatments and three replicates, differing in organic selenium content, Se0.5 (0.5 ppm), Se2 (2.0 ppm), Se4 (4.0 ppm), Se6 (6.0 ppm) and Se8 (8.0 ppm). Se4 achieved the highest final biomass at 2092.97g (P>0.05) and ADG at 34.79 g/day/aquarium (P<0.05). The superior performance was supported by favorable metabolic biomarkers, showing highest expression of the igf1 gene at 2.75 (P<0.05), avoided hyperglycemia with blood glucose maintained at 33.67mg/ dL (P<0.05), hepatosomatic index of 1.85 (P<0.05) and highest body selenium retention at 18.53% (P<0.05). Selenium supplementation exerted a positive effect on C. gariepinus energy metabolism. In conclusion, practical African catfish diets can implement the 4.0mg/ kg organic selenium supplementation regime to achieve optimum growth and enable cost-saving lower protein diets.

Aquaculture is a crucial food-producing sector that can supply more essential nutrients to nourish the growing human population. However, it faces challenges, including limited water quality and space competition. These constraints have led to the intensification of culture systems for more efficient resource use while maintaining or increasing production levels. However, intensification introduces stress risks to cultured organisms by, for instance, overcrowding, waste accumulation, and water quality deterioration, which can negatively affect the growth, health, and immunity of animals and cause diseases. Additionally, environmental changes due to climate and anthropogenic activities further intensify the environmental stress for aquaculture organisms, including crustaceans. Shrimp are one of the most widely cultured and consumed farmed crustacea. Relative to aquatic vertebrates such as fish, the physiology of crustaceans has simpler physiological structures, as they lack a spinal cord. Consequently, their stress response mechanisms follow a single pathway, resulting in less complex responses to stress exposure compared to those of fish. While stress is considered a primary factor influencing the growth, health, and immunity of shrimp, comprehensive research on crustacean stress responses remains limited. Understanding the stress response at the organismal and cellular levels is essential to identify sensitive and effective stress biomarkers which can inform the development of targeted intervention strategies to mitigate stress. This review provides a comprehensive overview of the physiological changes that occur in crustaceans under stress, including hormonal, metabolic, hematological, hydromineral, and phenotypic alterations. By synthesizing current knowledge, this article aims to bridge existing gaps and provide insights into the stress response mechanisms, paving the way for advancements in crustacean health management.

The objective of this research was to determine the optimum level of protein and lipid in improving the growth performance of Nile tilapia Oreochromis niloticus. This study was conducted using a 2 x 2 randomized factorial design with two treatment factors: the level of protein (28% and 32%) and the level of lipid (6% and 8%). So there were four test treatments and carried out with three replications. Nile tilapia with an average body weight of 5.60 ± 0.06 g were reared in the 900×45×35 cm aquarium at a density of 20 fish/aquarium. The experimental fish were reared for 60 days and fed three times daily to apparent satiation levels. Parameters evaluated in this study were growth performance and blood chemistry. The fish fed with diet 32;8 showed significantly higher growth than other treatments. Plasma protein in the fish fed with 32;6 diets were higher than those of plasma triglycerides, high density lipoprotein, and low density lipoprotein content were the same among the treatments. The result shows that feed 28;8 has the best protein efficiency ratio and protein retention. Keyword: Oreochromis niloticus, protein sparing effect, lipid, feed. ABSTRAK Tujuan penelitian ini adalah untuk mengetahui kadar protein dan lipid yang optimum dalam meningkatkan kinerja pertumbuhan ikan nila Oreochromis niloticus. Penelitian ini menggunakan rancangan acak faktorial 2 x 2 dengan dua faktor perlakuan yaitu kadar protein (28% dan 32%) dan kadar lipid (6% dan 8%). Jadi terdapat empat perlakuan uji dan dilakukan dengan tiga kali ulangan. Ikan nila dengan rata-rata bobot badan 5,60 ± 0,06 g dipelihara dalam akuarium berukuran 900×45×35 cm dengan kepadatan 20 ekor/akuarium. Ikan dipelihara selama 60 hari dan diberi makan tiga kali sehari secara at satiation. Parameter yang dievaluasi dalam penelitian ini adalah kinerja pertumbuhan dan kimia darah. Ikan yang diberi perlakuan dengan pakan 32;8 menunjukkan pertumbuhan yang lebih tinggi dibandingkan perlakuan lainnya. Protein plasma pada ikan yang diberi pakan 32;6 lebih tinggi dibandingkan dengan trigliserida plasma, HDL dan LDL rendah yang sama antar perlakuan. Hasil penelitian menunjukkan bahwa pakan 28;8 mempunyai nilai protein efesiensi rasio dan retensi protein terbaik. Keyword: Oreochromis niloticus, protein sparing effect, lemak, pakan

Feed is a crucial factor in tilapia farming because it contributes 92% of the total production cost. This study aims to evaluate the digestibility and utilization of carbohydrates in low-protein feed supplemented with NSP enzymes and organic chromium. The research method employed a factorial completely randomized design with treatments of organic chromium (0, 1, 2) and NSP enzymes (0, 1). The study used 18 aquariums, each measuring 50×40×35 cm. The fish used were tilapia with an average weight of 33.09 ± 0.06 g per fish. The feed used was commercial feed supplemented with organic chromium and NSP enzymes. The study focused on digestibility performance, digestive enzyme activity, growth performance, and antioxidant status. The results showed that the treatments E1C1 and E0C2 resulted in better total digestibility, energy digestibility, and protein digestibility compared to the control and other treatments. Lipase, protease, and amylase enzymes in tilapia fed with the E1C1 treatment showed significantly higher results (P0.05). However, antioxidant status, specifically SOD and GPx, was significantly higher in the E0C1 treatment compared to the control (P<0.05). The conclusion of this study is that the addition of NSP enzymes and organic chromium to low-protein feed can improve digestibility and optimize carbohydrate utilization, with the best results achieved with the E1C1 treatment. Keywords: antioxidant, digestive enzymes, Nile tilapia, nutrient digestibility ABSTRAK Pakan menjadi factor krusial dalam usaha budidaya ikan nila karena memiliki kontribusi sebesar 92% dari total biaya produksi. Penelitian ini bertujuan untuk mengevaluasi kecernaan dan kemampuanpemanfaatan karbohidrat pada pakan protein rendah yang diberienzim NSP dan kromium organik. Metode penelitian ini menggunakan rancangan acak lengkap faktorial dengan perlakuan kromium organic (0,1,2) dan enzim NSP (0,1). Wadah yang digunakan merupakan aquarium yang berukuran 50×40×35 cm sebanyak 18 unit. Ikan yang digunakan dalam penelitian ini adalah ikan nila dengan bobot rata-rata 33,09 ± 0,06 g/ekor. Pakan yang digunakan merupakan pakan komersial yang diberi penambahan kromium organik dan enzim NSP. Penelitian ini difokuskan pada kinerja kecernaan, aktivitas enzim pencernaan, kinerja Pertumbuhan serta status Antioksidan. Hasil penelitian menunjukkan bahwa perlakuan E1C1 dan E0C2 menghasilkan nilai kecernaan total, kecernaan energi dan kecernaan protein yang lebih baik dibanding perlakuan kontrol dan perlakuan lainnya. Enzim lipase, protease dan amilase pada ikan nila yang diberipakan perlakuan E1C1 menunjukkan hasil yang lebih tinggi secarasignifikan (P0,05). Walaupun demikian, status antioksidan yang dihasilkan yaitu SOD dan GPx pada perlakuan E0C1 menghasilkan nilai yang lebih tinggi secara signifikan terhadap kontrol (P<0,05). Kesimpulan dari penelitian ini adalah penambahan enzim NSP dan kromium organik pada pakan rendah protein mampu meningkatkan kinerja kecernaan dan memanfaatkan karbohidrat dengan optimal. Hasil terbaik pada perlakuan E1C1. Kata kunci: antioksidan, enzim pencernaan, kecernaan nutrient, ikan nila

This study aims to evaluate the effectiveness of NSP enzyme and organic chromium supplementation in low-protein Colosoma macropomum diet to increase carbohydrate utilization. The diets contains isoprotein (20%) and isoenergy. Factorial design with two factors, namely NSP enzyme (0 and 1 g/kg) and organic chromium (0, 1, and 2 mg/kg). Fish are reared in an aquarium measuring 80 x 50 x 35 cm3 and containing 75 L of water and stock at the density of 15 fish and reared for 60 days. The test parameters used are postprandial blood glucose levels, antioxidant activity, glycogen in muscle and liver, and growth performance. The results showed that the highest postprandial blood glucose levels were when the NSP enzyme was administered. Furthermore, the lowest malondialdehyde (MDA) values and the highest superoxide dismutase (SOD) and glutathione peroxide (GPx) values were obtained in fish fed feed containing 1 g/kg of NSP enzyme and 1 mg/kg organic chromium. Fish fed feed containing 1 g/kg NSP enzyme and 1 mg/kg organic chromium showed significant values in liver and muscle glycogen as well as growth performance compared to other treatments. From this research, it can be concluded that supplementation of NSP enzyme and organic chromium at doses of 1 g and 1 mg per kg of feed produces the best growth performance and anti-oxidant capacity in pomfret fish. Keywords: antioxidant, glycogen, growth performance, postprandial blood glucose ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efektivitas suplementasi enzim NSP dan kromium organik pada pakan ikan bawal Colosoma macropomum rendah protein untuk meningkatkan pemanfaatan karbohidrat. Pakan yang digunakan mengandung iso protein (20%) dan isoenergi. Rancangan faktorial dengan dua faktor yakni enzim NSP (0 dan 1 g/kg) dan kromium organik (0, 1, dan 2 mg/kg). Ikan bawal dipelihara dalam akuarium dengan ukuran 80 x 50 x 35 cm3 dan berisi air 75 L diisi 15 ekor ikan dan dipelihara selama 60 hari. Adapun parameter uji yang digunakan adalah pengukuran kadar glukosa darah postprandial, aktivitas antioksidan, glikogen, dan kinerja pertumbuhan. Hasil penelitian menunjukkan kadar glukosa darah postprandial tertinggi pada pemberian enzim NSP. Selanjutnya nilai malondialdehyde (MDA) terendah dan superoxide dismutase (SOD) dan glutathione peroxide (GPx) tertinggi didapat pada ikan yang diberi pakan mengandung pada 1 g/kg enzim NSP dan 1 mg/kg kromium organik. Ikan yang diberi pakan yang mengandung 1 g/kg enzim NSP dan 1 mg/kg kromium organik menunjukkan nilai signifikan pada glikogen hati dan otot serta kinerja pertumbuhan dibanding perlakuan lainnya. Dari penelitian ini dapat disimpulakan bahwa suplementasi enzyme NSP dan Kromium organik pada dosis 1 g dan 1 mg per kg pakan menghasilkan kinerja pertumbuhan dan kapasitas antioksidan terbaik pada ikan bawal. Kata kunci: antioksidan, glikogen, glukosa darah postprandial dan kinerja pertumbuhan

Micro minerals play a crucial role in the physiological processes of fish, acting as enzyme cofactors and stabilizing enzyme and protein structures. One of the essential micro minerals needed in the body is zinc (Zn). Zinc is utilized for various physiological processes, including the maintenance of enzymatic processes within the fish's body. The aim of this literature review is to analyze the optimal level of zinc supplementation in feed to enhance the growth performance of Nile tilapia (Oreochromis niloticus), based on the reported findings of conducted research. The literature sources were obtained from accredited national journals, international journals, research outcomes, theses, and dissertations over the past 20 years. The results of this literature review indicate that zinc supplementation in feed can have a positive impact on overall fish growth by increasing digestive enzyme activity, particularly protease enzymes. The level of zinc supplementation in fish is influenced by several factors including, fish size, feed consumption quantity, and duration of maintenance. The optimal dietary zinc intake for Nile tilapia growth is reported to be in the range of 20–50 mg/kg. Keywords: growth, literature study, Nile tilapia, zinc ABSTRAK Mikro mineral memiliki peran yang sangat penting dalam fisiologi tubuh ikan yang berfungsi sebagai kofaktor enzim dan menjadi penstabil struktur enzim maupun protein. Salah satu mikro mineral yang memiliki fungsi penting dan diperlukan dalam tubuh adalah unsur seng (Zn). Unsur Zn digunakan untuk proses fisiologis termasuk pemeliharaan proses enzimatik dalam tubuh ikan. Tujuan studi pustaka ini adalah menganalisis tingkat suplementasi Zn yang optimum dalam pakan untuk meningkatkan performa pertumbuhan ikan nila Oreochromis niloticus berdasarkan laporan hasil-hasil penelitian yang telah dilakukan. Sumber pustaka didapatkan dari jurnal nasional terakreditasi, jurnal internasional, hasil penelitian, tesis, dan disertasi pada kurun waktu 20 tahun terakhir. Hasil studi pustaka ini menunjukkan bahwa suplementasi Zn dalam pakan dapat memberikan pengaruh positif pada pertumbuhan ikan secara umum melalui peningkatan aktivitas enzim pencernaan terutama enzim protease. Tingkat suplementasi Zn pada ikan dipengaruhi oleh beberapa faktor diantaranya, ukuran ikan, jumlah konsumsi pakan, dan lama pemeliharaan. Nilai asupan Zn yang memberikan pertumbuhan optimum pada ikan nila adalah sebesar 20–50 mg/kg. Kata kunci: ikan nila, pertumbuhan, seng, studi pustaka