Guowen Dong

Here, we report the complete genome sequence of Anaerocolumna sp. MB42-C2 isolated from a hot spring in Qingliu, Fujian, China. The genome comprises 6,437,239 bp with a G + C content of 36.49 mol% and provides a genomic basis for further species delineation and characterization of genomic features.

Here, we report the complete genome sequence of Tissierella sp. strain MB52-C2 (= BCRC 81439) isolated from Wuyi Mountain soil, the source region of Wuyi rock tea. The genome is 3,892,263 bp with 30.9% G + C content and supports species delineation and genomic analyses of taxonomic position and genomic features.

A microbial community study using a culture-dependent method was conducted on dehydrated sludge collected from a steel factory’s wastewater treatment plant. One isolate, designated QWL-01^T, was a strictly anaerobic, Gram-stain-negative, non-motile, non-spore-forming bacterium with coccoid cells measuring 0.6–0.9 μm in diameter. The growth of strain QWL-01^T was observed at 4–40 °C (optimum at 28–35 °C), pH 5.5–8.0 (optimum at pH 7.1), and a range of 0–3% NaCl (optimum at 0.5%). An analysis of the Biolog AN plate revealed positive carbon source utilization only for palatinose, α-ketovaleric acid, and pyruvic acid. The predominant fatty acids were iso-C_13:0 (17.0%), C_16:0 dimethyl acetal (12.0%), and anteiso-C_13:0 (9.2%). A 16S rRNA gene sequence analysis through BLASTN demonstrated that the nearest phylogenetic neighbors of the novel strain were Youngiibacter multivorans DSM 6139^T (93.82%) and Proteiniclasticum ruminis JCM 14817^T (93.75%). The genome size of strain QWL-01^T was 3.69 Mbp, with a G+C content of 50.8 mol%. Comparing strain QWL-01^T with closely related species of genera Proteiniclasticum and Youngiibacter, the digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI), and average amino acid identity (AAI) values ranged from 26.60% to 36.80%, 65.89% to 68.30%, and 49.27% to 51.58%, respectively. Based on phenotypic, physiological, phylogenetic, and genomic relatedness evidence, strain QWL-01^T represents a novel genus in the family Clostridiaceae, for which the name Anaerococcoides asporogena gen. nov. sp. nov. is proposed. Strain QWL-01^T (=BCRC 81396^T = CICC 25258^T = NBRC 117088^T) is the type strain of the proposed novel species.

Here, we report the complete genome sequence of Oscillospiraceae bacterium MB24-C1 (= BCRC 81428), isolated from sewage sludge of the Sanming Steel wastewater treatment plant, China. The genome comprises 2,952,321 bp with a GC content of 48.1% and was used for further species delineation and comparative genomic analyses.

Rechargeable aqueous zinc-ion batteries (AZIBs) are safe and affordable energy storage devices. However, AZIBs face significant challenges such as zinc dendrite formation, electrode passivation, and corrosion. Hydrogen-bonded organic frameworks (HOFs), the crystalline porous materials self-assemble via intermolecular complementary hydrogen bonds, can enhance ion exchange rates and thereby improve battery performance. In this study, HOF-DAT is synthesized and introduced to modify glass fiber (GF) separators (HOF-GF). The HOF-GF separator enhances the reversibility of zinc electrochemistry during cycling, alleviating degradation associated with nonuniform metal growth. In Zn//Zn symmetric cells, stable operation is maintained for prolonged cycling at 1 mA cm ⁻² with an areal capacity of 1 mAh cm ⁻² . These cells also exhibit consistent performance under higher current densities, maintaining stable operation even at 5 mA cm ⁻² . Additionally, Zn//NVO batteries assembled with HOF-GF separators exhibit a high initial capacity of 430 mAh g⁻ ¹ at 0.1 A g⁻ ¹ , and improve capacities at different currents. By demonstrating a scalable separator engineering concept, this study advances zinc anode regulation while offering a viable pathway toward durable and economically competitive aqueous zinc-ion battery systems.

Here, we report the complete genome sequence of Bacteroidales bacterium strain MB20-C3-3 (=BCRC 81427) isolated from sewage sludge. The genome is 2,379,874 bp in length with a guanine and cytosine content of 40.0%. The genome of strain MB20-C3-3 was used for further species delineation and comparative genomic analyses.

The gut microbiota of Tenebrio molitor has attracted attention for its potential in solving plastic waste problems. Here, we report the complete genome sequence of Propionimicrobium sp. PCR01-08-3, isolated from the feces of Tenebrio molitor larvae. The genome of strain PCR01-08-3 was selected for further species delineation and comparative genomic analysis.

To date, only a few microbial community studies of cold seeps at the South China Sea (SCS) have been reported. The cold seep dominated by tubeworms was discovered at South Yungan East Ridge (SYER) offshore southwestern Taiwan by miniROV. The tubeworms were identified and proposed as Paraescarpia formosa sp. nov. through morphological and phylogenetic analyses. The endosymbionts in the trunk of P. formosa analyzed by a 16S rRNA gene clone library represented only one phylotype, which belonged to the family Sedimenticolaceae in Gammaproteobacteria. In addition, the archaeal and bacterial communities in the habitat of tubeworm P. formosa were investigated by using high-phylogenetic-resolution full-length 16S rRNA gene amplicon sequencing. The results showed that anerobic methane-oxidizing archaea (ANME)-1b was most abundant and ANME-2ab was minor in a consortia of the anerobic oxidation of methane (AOM). The known sulfate-reducing bacteria (SRB) partners in AOM consortia, such as SEEP-SRB1, -SRB2, and -SRB4, Desulfococcus and Desulfobulbus, occurred in a small population (0–5.7%) at the SYER cold seep, and it was suggested that ANME-1b and ANME-2ab might be coupled with multiple SRB in AOM consortia. Besides AOM consortia, various methanogenic archaea, including Bathyarchaeota (Subgroup-8), Methanocellales, Methanomicrobiales, Methanosarcinales, Methanofastidiosales and Methanomassiliicoccales, were identified, and sulfur-oxidizing bacteria Sulfurovum and Sulfurimonas in phylum Epsilonbacteraeota were dominant. This study revealed the first investigation of microbiota in and around tubeworm P. formosa discovered at the SYER cold seep offshore southwestern Taiwan. We could gain insights into the chemosynthetic communities in the deep sea, especially regarding the cold seep ecosystems at the SCS.

We report the complete genome sequence of Tissierella sp. strain Yu-01 (=BCRC 81391), isolated from the feces of black soldier fly ( Hermetia illucens ) larvae. This fly has increasingly been gaining attention because of its usefulness for recycling organic waste. The genome of strain Yu-01 was selected for further species delineation.