Mengyao Li
Research Subjects
Mengyao Li has a total of 5 co-authors. Here are the top co-authors by number of shared publications:
- Chen Zhai
COFCO Nutrition and Health Research Institute, Beijing Key Laboratory of Nutrition and Health and Food Safety, Beijing 102209, PR China
1 shared publication Shuya Wang
Nutrition & Health Research Institute, COFCO Corporation, Beijing Key Laboratory of Nutrition & Health and Food Safety, Beijing 102209, China
1 shared publicationWeixia Huang
Nutrition & Health Research Institute, COFCO Corporation, Beijing Key Laboratory of Nutrition & Health and Food Safety, Beijing 102209, China
1 shared publicationYunguo Liu
College of Life Science and Technology, Xinjiang University, Urumqi 830002, China
1 shared publication- Zhao Li
Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, and Shaanxi Key Laboratory for Hazard Factors Assessment in Processing and Storage of Agricultural Products, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China
1 shared publication
Latest Publications
- Open Access
- 9 December 2019
- Published by Royal Society of Chemistry (RSC) in RSC Advances
- Vol. 9 (69) , 40689-40693
Detection of carboxylesterase by a novel hydrosoluble near-infrared fluorescence probe
A novel hydrosoluble near-infrared fluorescence off–on probe has been developed for detecting carboxylesterase activity. The probe was designed by introducing (4-acetoxybenzyl)oxy as a quenching and recognizing moiety to the decomposed product of IR-783, which exhibits excellent near-infrared fluorescence features and good water solubility. The responding mechanism of novel probe 1 to carboxylesterase was investigated. It would lead to the cleavage of the carboxylic ester bond by carboxylesterase catalyze the spontaneous hydrolysis of the probe, resulting in the release of a near-infrared fluorophore. This behaviour leads to the development of a simple and sensitive fluorescent method for assaying carboxylesterase activity, with a detection limit of 3.4 × 10−3 U mL−1. Moreover, the probe displays excellent selectivity toward carboxylesterase over other substances. Notably, the imaging experimental results showed that the probe 1 is cell membrane permeable, and its applicability has been demonstrated for monitoring carboxylesterase activity in HeLa cells.
- 9 December 2019
- Published by Royal Society of Chemistry (RSC) in RSC Advances
- Vol. 9 (69) , 40689-40693
Open AccessDetection of carboxylesterase by a novel hydrosoluble near-infrared fluorescence probe
A novel hydrosoluble near-infrared fluorescence off–on probe has been developed for detecting carboxylesterase activity. The probe was designed by introducing (4-acetoxybenzyl)oxy as a quenching and recognizing moiety to the decomposed product of IR-783, which exhibits excellent near-infrared fluorescence features and good water solubility. The responding mechanism of novel probe 1 to carboxylesterase was investigated. It would lead to the cleavage of the carboxylic ester bond by carboxylesterase catalyze the spontaneous hydrolysis of the probe, resulting in the release of a near-infrared fluorophore. This behaviour leads to the development of a simple and sensitive fluorescent method for assaying carboxylesterase activity, with a detection limit of 3.4 × 10−3 U mL−1. Moreover, the probe displays excellent selectivity toward carboxylesterase over other substances. Notably, the imaging experimental results showed that the probe 1 is cell membrane permeable, and its applicability has been demonstrated for monitoring carboxylesterase activity in HeLa cells.