Maria Rosa Santos Breda

To evaluate whether the joint use of autologous platelet-rich plasma (aPRP) and rosuvastatin (RSV) in biopsies of dermal wounds induced in rabbits results in an additive effect on the immunoexpression of collagens type I and III, optimizing the healing process and increasing collagen production during the proliferative phase of healing to improve the quality of tissue repair. Thirty-two biopsy samples from eight clinically healthy adult male New Zealand rabbits were used. They were treated with aPRP, RSV, or aPRP + RSV and analyzed zero, three, seven, ten, and 14 days post wound induction. Type I collagen immunoexpression was significantly higher in wounds treated with aPRP when compared to the control group. This study demonstrated that type III collagen is predominant during the proliferation phase of the healing process, highlighting its critical role in tissue repair and regeneration. The association of aPRP and RSV in wound treatment may have an additive effect in the immunoexpression of type III collagen and can thus be used as an alternative in tissue repair and collagen formation, optimizing the healing process.

Background: Cholelithiasis is a digestive system disorder of multifactorial origin that occurs due to stones formed in the gallbladder. This study aimed at investigating by abdominal ultrasound examination the prevalence of cholelithiasis in lion tamarins kept under human care. Methods: Thirty lion tamarins from five Brazilian zoos, including 17 golden lion tamarins and 13 golden-headed lion tamarins, were evaluated. Results: Considering all lion tamarins, the overall frequency of cholelithiasis was 53.3% (16/30). There were no significant differences between species and sex. Cholelithiasis was predominant (75.0%) in lion tamarins older than 5 years. Septate gallbladder was observed in 86.6% (26/30) of the lion tamarins. Of these, 53.8% (14/26) had gallstones. Biochemical analysis revealed a moderate positive correlation between gamma-glutamyl transferase and the number of gallstones. Conclusions: Asymptomatic cholelithiasis is frequent in lion tamarins kept under human care. Therefore, systematic monitoring through ultrasound should be part of the preventive care of these animals.

Estudos têm sido realizados para verificar os efeitos de biomateriais, como o plasma rico em plaquetas (PRP) e a fibrina rica em plaquetas (FRP) e a sua contribuição para o processo de cicatrização de feridas por meio da liberação de fatores de crescimento que têm propriedades de indução de regeneração tecidual. Muitas evidências também se mostram favoráveis ao uso tópico da rosuvastatina (RSV) em lesões de pele, por modificar de forma positiva o fluxo sanguíneo basal tecidual. No entanto, não está claro se este efeito está envolvido no aumento do fluxo sanguíneo e por consequência uma melhora no processo cicatricial. Neste sentido, o presente estudo analisou a quantidade de fibroblastos e número de vasos em feridas induzidas experimentalmente em coelhos após tratamento com e sem associação dos biomateriais e RSV. A hipótese baseia-se que o uso tópico de um fármaco hipolipemiante associado a um biomaterial possa aumentar a quantidade de fibroblastos e angiogênese após lesão experimentalmente induzida, e com isso aumentando a colagenização para produção de uma cicatriz mais homogênea. Verificou-se que a associação da FRP e RSV promoveu um efeito aditivo aumentando em 135% a angiogênese. O uso da RSV isolada também proporcionou um aumento de 77% na quantidade de fibroblastos. Concluiu-se que tanto a FRP quanto a RSV possuem bons efeitos cicatrizantes, de tal forma que a associação entre elas foi benéfica e demonstrou um possível efeito aditivo.

Platelet-rich plasma (PRP) has been extensively studied as a biomaterial for wound treatment, and the heterologous PRP is usefulin the event that obtaining the patient’s own blood is impossible. This study aimed to evaluate and compare wound healing in rabbits and quantify the collagen in experimentally induced wounds in a control group and in a group treated with heterologous PRP gel. We hypothesize that this gelis capable of promoting proper healing with no adverse reactions, increased collagen content. The clinical aspects of coloring, edema, hyperemia, exudation, crust, granulation, pain sensitivity, and retraction index of the wounds were measuredon days 7, 14, and 17 after the injury. Collagen quantification by Picrosirius staining and evaluation under polarized light was performed on the 17th day. Crust was present in both groups at all evaluated time points, with the absence of other clinical signs. The wound contraction rate and collagen quantity did not differ between groups. In conclusion, the suggested hypothesis was partially confirmed; the heterologous PRP gel was unable to increase the amount of collagen and accelerate the wound healing process, however, wound healing was efficient and similar in both groups and there was no local adverse reaction. Thus, despite the scarcity of studies in the literature, the heterologous PRP gel is an effective alternative treatment for wounds in the absence of other sources of PRP.