Sujith Somasekharan Nair

The primary aim of the study is to develop creams containing herbal extract and assess their effectiveness. The utilization of natural products is of great significance due to concerns regarding the toxicity and side effects associated with modern drugs. The objective of this research is to formulate sun protection creams that are cost-effective, non-toxic, and efficacious, and to ascertain if these formulations are as effective as those already on the market. The study on skin moisturization re

Background: An increasing number of people are turning to herbal medicines in their search for innovative pharmaceuticals since they are effective treatments for a wide variety of conditions and traditional herbs are rich in bioactive chemicals. In this study, we looked at whether or not a petiole extract of Eichhornia crassipes preserved in methanol inhibited the proliferation of prostate cancer (PC3) cell lines. Materials and methods: Lakes in Ezhikkara, Ernakulum, Kerala, were the source of E. crassipes. Soxhlet extraction was used to create the extract. 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cell viability of methanolic petiole extract at various concentrations. Mean and standard deviation was used to determine absorbance scores. Utilizing probit analysis, we determined the IC50 value. The descriptive statistics to measure the percent of viable cells along with the regression equation were calculated using SPSS. Results: It has been shown that the methanol extract significantly impacted PC3 cell lines' capacity to survive. It was also determined that increasing the medication concentration resulted in a decrease in cell viability. The percentage of living cells was measured after being exposed to methanol extracts at concentrations of 12.5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, and 200 μg/ml, and found to be 95.13, 85.88, 76.12, 64.33, and 53.62 percent, respectively. With IC50 values of 199.488 g/ml, it was shown that methanolic petiole extracts of E. crassipes are cytotoxic. Using probit analysis, we determined that the regression equation is y = -0.2051x + 90.915, with an R2 value of 0.893. Conclusion: As a result of its chemotherapeutic properties, the E. crassipes petiole extract has the potential to be employed in therapeutic applications, with the ultimate goal of bettering prostate cancer management practices and clinical results by drastically lowering cell viability. The study's results may pave the way for fresh chemotherapeutic approaches to be developed for the treatment of androgen-independent prostate cancer.

Eichhornia crassipes (E. crassipes) have several secondary metabolites that have medicinal value. These include sterols, alkaloids, phenolics, flavonoids, tannins, and saponins. In the current study, the methanolic petiole extract of E. crassipes was examined to determine its potential antiproliferative activity against Sloan Kettering Melanoma 5 (SK-Mel-5) cell lines. Eichhornia crassipes were obtained from the water bodies of Ezhikkara, Ernakulam, Kerala. The Soxhlet technique was used to produce the extract. Leaves, petioles, and roots were dried and pulverized before being analyzed phytochemically in a number of solvents. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure the extent to which various dosages of the extract inhibited cell proliferation, and the methanolic extract of petioles was chosen since it contained more anticancer components. The half maximal inhibitory concentration (IC₅₀) was determined by utilizing a probit model and the slope-gradient method of the regression equation. The Statistical Package for Social Sciences (SPSS) version 21 (IBM SPSS Statistics, Armonk, NY) was used for the analysis. We examined the effects of 12.5, 25, 50, 100, and 200 μg/ml of methanolic petiole extract. The data indicated that the methanol extract significantly reduced SK-Mel-5 cell viability. Cell growth inhibition increased with concentration but was shown to be relatively low at 100 g/ml, exhibiting 38.911% of inhibitory activity. The percentage of cell growth inhibition at 200 g/ml was 52.965%. The methanolic petiole extracts of E. crassipes were found to be cytotoxic with IC₅₀ values of 172.186 g/ml. Probit analysis was performed to obtain the regression equation. The in vitro study suggests that the methanol extract of the petiole of E. crassipes had modest antiproliferative action against SK-Mel-5 cells, a typical human melanocyte tumor cell line. The study findings shed light on the anticancer activity of E. crassipes, making it an appropriate source of drug-lead chemicals for the development of safer and cost-effective remedies for cutaneous ailments varying from rashes to awful melanoma.