Marta Olga Krzyżanowska

In recent years, there has been a dynamic development in therapies utilizing extracellular vesicles (EVs) including exosomes. Therefore, we have conducted an analysis of the scientific literature to verify the current state of knowledge about these therapies. A total of 12 clinical studies were analyzed, covering the use of EVs in treating skin aging, acne scars, alopecia, and wound healing. The results indicate that EVs and exosomes hold potential in regenerative skin therapies, offering innovative and non-invasive therapeutic approaches. At the same time, significant challenges related to the standardization of their production and the lack of large-scale randomized studies were identified. Thus, we also evaluated the investigated clinical trials in regard to the MISEV (Minimal Information for Studies of Extracellular Vesicles) criteria. This review provides a comprehensive overview of the contemporary applications of EVs in skin therapy and regenerative medicine, highlighting directions for further research.

Our review aimed to assess the effects of bariatric surgery-induced weight loss on ocular functions. We focused on retinochoroidal microcirculation, glaucomatous factors, and the condition of the eye surface pre- and postoperatively. The review covered 23 articles, including five case reports. Bariatric surgery positively impacts retinochoroidal microcirculation. The arterial perfusion and vascular density improve, venules constrict, and the arteriole-to-venule ratio increases. Weight loss positively correlates with intraocular pressure decrease. The impact of postoperative weight loss on the choroidal thickness (CT) and the retinal nerve fiber layer (RNFL) is still unclear. The correlation between ocular symptoms and hypovitaminosis A needs to be evaluated. Further research is required, especially regarding CT and RNFL, mainly focusing on long-term follow-up.

Effective wound management is an important determinant of the survival and prognosis of patients with severe burns. Thus, novel techniques for timely and full closure of full-thickness burn wounds are urgently needed. The purpose of this review is to present the current state of knowledge on the local treatment of burn wounds (distinguishing radiation injury from other types of burns) with the application of cellular therapies conducted in clinical studies. PubMed search engine and ClinicalTrials.gov were used to analyze the available data. The analysis covered 49 articles, assessing the use of keratinocytes (30), keratinocytes and fibroblasts (6), fibroblasts (2), bone marrow-derived cells (8), and adipose tissue cells (3). Studies on the cell-based products that are commercially available (Epicel^®, Keraheal™, ReCell^®, JACE, Biobrane^®) were also included, with the majority of reports found on autologous and allogeneic keratinocytes. Promising data demonstrate the effectiveness of various cell-based therapies; however, there are still scientific and technical issues that need to be solved before cell therapies become standard of care. Further evidence is required to demonstrate the clinical efficacy and safety of cell-based therapies in burns. In particular, comparative studies with long-term follow-up are critical.

Aim. The aim of this study was to obtain information, whether patients with heart failure (HF) follow medical indications and if there are any sociodemographic differences between compliant and noncompliant patients and how lifestyle changes contribute to the patients’ wellbeing.Material and Methods. A survey was carried out in 198 patients hospitalized in I Clinic of Cardiology in Poznan. Patients were divided into groups: P1- no lifestyle changes, P2- 1 of 4 verified lifestyle modifications, P3- 2 or 3 verified lifestyle modifications, P4- all 4 verified lifestyle modifications.Results. People over the age of 65 mostly constitute groups of low compliance (p = 0,055). Men are more eager to change their lifestyle (91,4% in P3 and 85,7% in P4). Women belong mainly to groups with low compliance (p = 0,0001). Respondents who reported condition improvement were less likely to describe decreased sexual activity (59,6% vs 38,2%, p = 0,078), which acted as an important determinant of family relations. However, our analysis demonstrated a non-significant impact of adherence to indicated lifestyle modifications on patients’ wellbeingConclusions. Compliant patients are statistically younger and more often male. Level of education is the least important determinant of compliance. There is no significant impact of adherence to indicated lifestyle modifications on patient’s wellbeing. Compliant patients are less likely to reduce their sexual activity, which showed to be an important family relationship factor.