Sri Rahayu

M ea t ten d eriz a ti o n t e c h ni q u e s o ft en inv o lv e ti m e-c o nsu m ing pr o c e ss e s o r c hem ic a l add itiv e s t ha t ma y r a is e hea lt h c o nc er ns o r a lter t he t as t e o f mea t. Expl o r i ng a ltern a tiv e n a tur a l me t hod s, suc h as utilizing proteolytic enzymes derived from moringa, presents an opportunity to address these limitations. Studies on moringa have been limited, especially those focusing on goat meat tenderization, leaving a gap in understanding how moringa affects this process. The research aims to bridge this gap by systematically examining how protease enzymes from moringa leaves can tenderize goat meat. The research was performed on goat meat samples (100 g of meat per sample) treated with the moringa leaves extract (prepared from 20 g of moringa leaves with 60 ml of distilled water) at different storage times (2, 24, and 46 hours), as well as a control sample without any additive. The results showed that the moringa leaves extract did not significantly alter pH values and increased cooking loss for the 24-hour treatment (44.3 %) and 46-hour treatment (39.8 %) compared to the control (36.8 %). Color analysis showed increased lightness (L*) and redness (a*). The yellowness (b*) showed considerable difference between the control and the 24-hour samples compared with the 2-hour and 46-hour samples. The moringa leaves extract significantly reduced tenderness by lowering hardness, gumminess, resilience, and chewiness. This research advances environmentally friendly, renewable solutions in the food industry because the food industry produces significant quantities of meat by-products and waste during processing. This research will reduce waste by transforming tougher or less desirable meat cuts into more tender and valuable products.

This study evaluated the effects of waru (Hibiscus tiliaceus) leaf meal (WLM) and bamboo (Gigantochloa apus) leaf meal (BLM) supplementation on energy metabolism and performance of local sheep fed on ammoniated rice straw and concentrate. Sixteen male local sheep aged 12–18 months with initial average body weight of 27.1 ± 2.11 kg (7.78% coefficient of variation) were completely randomized according to a Completely Randomized Design (CRD) to receive one of the following treatments: T0 (ammoniated rice straw + concentrate feed); T1 (T0 + 2.49 g WLM); T2 (T0 + 1.87 g WLM + 0.33 g BLM); and T3 (T0 + 1.25 g WLM + 0.65 g BLM). The concentrate was provided at 3% of live weight, and ammoniated rice straw was available on a controlled ad libitum. Analysis of variance indicated that WLM and BLM supplementation significantly increased digestible energy (DE), metabolizable energy (ME), energy retention (ER), ER to EI efficiency, ER to DE efficiency, average daily gain (ADG), and consumption rate (CR), while significantly decreasing the feed conversion ratio (FCR) (P0.05) on dry matter intake (DMI) and energy intake (EI). In conclusion, dietary supplementation with 1.87 g of WLM and 0.33 g of BLM per kg concentrate was more effective than the other combinations in improving energy metabolism and performance of local sheep.

The Black Soldier Fly (BSF; Hermetia illucens) larvae are recognized for their ability to convert diverse organic materials into protein-rich biomass, depending on the substrate they consume. The composition of these substrates can significantly impact the nutrient profile and enzyme activities of the resulting maggot protein extract (MPE). Therefore, this exploratory research aimed to assess the nutrient content, protease, and cellulase activity of MPE obtained from BSF maggots reared on different substrates, with a specific focus on substrates A (comprising restaurant waste and rejected milk), B (layer manure), and C (kitchen waste). The results showed that maggot meal from layer manure had the highest protein content (45.36%) and the lowest fat content (18.44%). Amino acids in maggot meal contained high levels of glutamic acid, aspartic acid, alanine, valine, leucine, and isoleucine. Lauric acids were found in maggot meal from kitchen waste (33.79%), layer manure (32.18%), and restaurant waste and rejected milk (22.94%). Maggot meal from layer manure had the highest oleic acid content (15.13%). The protein concentration of MPE from various substrates ranged from 0.56 to 0.601 mg/ml (at 60% w/v ammonium sulfate saturation) and 0.555 to 0.609 mg/ml (at 70% ammonium sulfate saturation). The protease activity of MPE from layer manure substrates exhibited optimum activity and stability in neutral to alkaline pH, with activity levels of 0.748 U/mg at pH 7.0 and pH 11.0 (at 60% w/v ammonium sulfate saturation) and 0.774 units/mg at 70% w/v ammonium sulfate saturation. The highest cellulase activity was found in MPE from kitchen waste, which remained stable at pH 5.0-11.0. In general, maggots from different substrate sources exhibited distinct nutrient profiles and enzyme activities. Protein extract from maggots grown in layer manure showed the most suitable nutrient profile for use as an alternative source of protein feed and protease enzymes.

The aim of this study was to evaluate the effect of Waru Leaf Flour (WLF) and Direct-Fed Microbials (DFM) supplementation on volatile fatty acid (VFA) and ammonia (N-NH3) concentrations, total protozoa, microbial protein synthesis (MPS), and the specific activity of cellulase, amylase, protease in rumen fluids of local beef cattle. The study was conducted as an experimental investigation using a factorial Completely Randomized Design (FCRD). The first factor were three levels of DFM (P) mixed with rice straw (0%, 0.5%, and 1% dry matter of rice straw). The second factor consisted of three levels of WLF supplementation (D) in concentrates (0%, 0.24%, and 0.48% of dry matter concentrate). This resulted nine treatment groups, each it replicated three times.The diet composition consisted of rice straw and concentrate at 40:60 ratio (% dry matter). The interaction between DFM and WLF had a statistically significant impact (p0.05) in relation to VFA, MPS, cellulase, amylase, and protease activity. While no significant interaction were observed for total VFA, cellulase, amylase, and protease, DFM supplementation had a statistically significant effect (P<0.01) on reducing enzymes activity, and total VFA (P<0.05). SDS-PAGE and zymography analyzes showed two protease molecules at all treatments with molecular weight of 144 kDa and 133 kDa, cellulase molecules at 62-67 kDa and 19-21 kDa respectively. The addition of DFM showed a new protease band, they had a measurement of 14 and 25 kDa.This study provides valuable insights into enzyme activities in rumen, particularly protease, cellulase, and amylase. The results suggest that the combination of 0.24% WLF and 0.5% DFM supplementation holds promise for optimizing rumen conditions and improving the performance of local beef cattle.

The aim of this study was to evaluate the supplementation of chicken claw-derived bioactive peptides on performance, carcass weight, total intestinal bacteria, and feed dry matter (DMD) and organic matter (OMD) digestibility of broilers. A completely randomized design and five repetitions were applied in this experiment. The research material consisted of 200 DOC strain CP-707 grown up to 35 days of age, and the biopeptide was produced by hydrolyzing chicken claws protein with a commercial papain enzyme. Bioactive peptides were added to feed treatments in amounts of 0, 2, 4, and 6%. The differences between treatments were tested using the honestly significant difference test. The addition of chicken claws biopeptides had a significant influence (P<0.01) on OMD and carcass weight, as well as a significant effect (P<0.05) on body weight gain, feed efficiency, DMD and total bacteria. Addition of chicken claws-derived peptides in rations up to 6% enhanced body weight gain, feed efficiency, carcass weight, DMD, OMD, and total intestinal bacteria in broiler chickens. Keywords: Biopeptides, Chicken, Claw, Digestibility, Carcass quality.

This study aimed to determine the nutrient content and rumen fermentation product in-vitro of rice straw from rice of the IR variety fertilized using organic and inorganic fertilization. This research used experimental methods. This study examined two paddy fields located in Datar Village, Sumbang District, Banyumas. Land A used inorganic fertilizer (urea and phonska) and Land B used organic fertilizer from cattle dung. Both of those lands were planted with IR variety rice. Samples were 20 points on each land obtained by random sampling so that all parts of the field were represented. All samples were dried in the oven at 600C. Variables measured were nutrient contents such as crude protein (CP), crude fiber (CF) and rumen fermentation product in-vitro of volatile fatty acids (VFA) and ammonia nitrogen (N-NH3 ). Statistical analysis used t-test. Result showed that CP and CF contents and rumen fermentation products of VFA of rice straw from organic fertilizer were higher (P0.05) in the rumen fermentation product of N-NH3 between organic and inorganic fertilizer. It can be concluded that rice straw quality from IR variety rice can be improved through organic fertilization. Keywords: organic, fertilizer, rumen, nutrient, rice straw

Objective: The objective of this study was to identify polymorphism in olfactomedin like 3 (OLFML3) gene, and association analysis with meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep, and expression quantification of OLFML3 gene in phenotypically divergent sheep.Methods: A total of 328 rams at the age of 10 to 12 months with an average body weight of 26.13 kg were used. A novel polymorphism was identified using high-throughput sequencing in sheep and genotyping of OLFML3 polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 328 rams, 100 rams representing various sheep genotypes were used for association study and proc general linear model was used to analyse association between genotypes and phenotypic traits. Quantitative real-time polymerase chain reaction (qRT-PCR) was used for the expression analysis of OLFML3 mRNA in phenotypically divergent sheep population.Results: The findings revealed a novel polymorphism in the OLFML3 gene (g.90317673 C>T). The OLFML3 gene revealed three genotypes: CC, CT, and TT. The single nucleotide polymorphism (SNP) was found to be significantly (p<0.05) associated with meat quality traits such as tenderness and cooking loss; carcass characteristics such as carcass length; retail meat cut such as pelvic fat in leg, intramuscular fat in loin and tenderloin, muscle in flank and shank; fatty acids composition such as tridecanoic acid (C13:0), palmitoleic acid (C16:1), heptadecanoic acid (C17:0), ginkgolic acid (C17:1), linolenic acid (C18:3n3), arachidic acid (C20:0), eicosenoic acid (C20:1), arachidonic acid (C20:4n6), heneicosylic acid (C21:0), and nervonic acid (C24:1). The TT genotype was associated with higher level of meat quality, carcass characteristics, retail meat cut, and some fatty acids composition. However, the mRNA expression analysis was not different among genotypes.Conclusion: The OLFML3 gene could be a potential putative candidate for selecting higher quality sheep meat, carcass characteristics, retail meat cuts, and fatty acid composition in sheep.

Native beef cattle breeds in Indonesia such as Bali Timor (BT), Bali Flores (BF), Madura (M), and Sumba Ongole (SO) come from different regions with different types of feed because of the different environmental ecosystems. This situation results in native cattle breeds having different microbial compositions and functions. The purpose of this study was to find native cattle breeds fed ammoniated rice straw and concentrate having the highest productivity and feed efficiency. The material used was four native beef cattle breeds with a weight of 210-250 kg and age of 3.5-3.7 years. There were ten for each native cattle and they were fed concentrate and ammoniated rice straw. The amount of concentrate fed was 2% of body weight, while ammoniated rice straw was prepared ad libitum. Randomized Block Design with an initial body weight of cattle as a group was used in this research. Covariance analysis showed that the breed of native cattle had a significant effect (P < 0.05) on average daily gain (ADG), feed conversion ratio (FCR), feed efficiency (FE), digestibility of dry matter (DMD), organic matter (OMD), neutral detergent fiber (DNDF), acid detergent fiber (DADF) and gross energy (DGE). BF cattle were higher (P 0.05). In contrast to nutrient digestibility, ADG of SO and M cattle were higher (P < 0.05) followed by BF and BT cattle, respectively. The FC of BT and BF cattle were similar (P > 0.05), but it was high (P < 0.05) compared to M and SO cattle. The conclusion is SO and M cattle have good ability when fattened using ammoniated rice straw and concentrate.